Calcineurin-mediated GABAA receptor dephosphorylation in rats after kainic acid- induced status epilepticus  Aihua Wang, Zhaofu Chi, Shengjun Wang, Shuhua Wang, Qinjian Sun  Seizure - European Journal of Epilepsy  Volume 18, Issue 7, Pages 519-523 (September 2009) DOI: 10.1016/j.seizure.2009.05.001 Copyright © 2009 British Epilepsy Association Terms and Conditions

Fig. 1 Changes in CaN phosphatase activity after KA-induced SE. (A) Basal and maximal CaN activity levels after SE. (A′) The effect of FK506 on CaN activity. When FK506 was administered prior to KA injection, CaN activity was significantly decreased at 2h after SE. Bars indicate averages±SD. *p<0.05, **p<0.01 vs. control (basal). #p<0.05, ##p<0.01 vs. control (max.). &&p<0.01 vs. KA and KA+saline (basal). ++p<0.01 vs. KA and KA+saline (max.). Seizure - European Journal of Epilepsy 2009 18, 519-523DOI: (10.1016/j.seizure.2009.05.001) Copyright © 2009 British Epilepsy Association Terms and Conditions

Fig. 2 Immunoblot analysis of CaN protein expression. (A) Immunoblot showing CaN protein expression (64kD). (A′) Western blot data were densitometrically quantified and normalized to an internal standard. Bars indicate averages±SD. *p<0.05, **p<0.01 vs. control. Seizure - European Journal of Epilepsy 2009 18, 519-523DOI: (10.1016/j.seizure.2009.05.001) Copyright © 2009 British Epilepsy Association Terms and Conditions

Fig. 3 The effect of FK506 on CaN protein expression at 72h after SE. (A) Immunoblot showing the effect of FK506 on CaN protein expression. (A′) Western blot data were densitometrically quantified and normalized to an internal standard. Bars indicate averages±SD. **p<0.01 vs. control; #p<0.05 vs. KA and KA+saline. Seizure - European Journal of Epilepsy 2009 18, 519-523DOI: (10.1016/j.seizure.2009.05.001) Copyright © 2009 British Epilepsy Association Terms and Conditions

Fig. 4 Immunoblot analysis of phosphorylation of serine/threonine residues in GABAAR 2/3 subunits. (A) Immunoblot showing phosphorylation of serine/threonine. (A′) Western blot data were densitometrically quantified and normalized to an internal standard. Bars indicate averages±SD. *p<0.05, **p<0.01 vs. control. Seizure - European Journal of Epilepsy 2009 18, 519-523DOI: (10.1016/j.seizure.2009.05.001) Copyright © 2009 British Epilepsy Association Terms and Conditions

Fig. 5 The effect of FK506 on phosphorylation of serine/threonine residues in GABAAR 2/3 subunits at 2h after SE. (A) Immunoblot showing the effect of FK506 on phosphorylation of serine/threonine residues in GABAAR 2/3 subunits. (A′) Western blot data were densitometrically quantified and normalized to an internal standard. Bars indicate averages±SD. *p<0.05, **p<0.01 vs. control; #p<0.05 vs. KA and KA+saline; &p<0.05 vs. KA and KA+saline. Seizure - European Journal of Epilepsy 2009 18, 519-523DOI: (10.1016/j.seizure.2009.05.001) Copyright © 2009 British Epilepsy Association Terms and Conditions