Melissa Apodaca Dr. A. Wijte CSULB

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Presentation transcript:

Melissa Apodaca Dr. A. Wijte CSULB Oxygen Production by Sedimentary Microalgae in a Natural and Constructed Salt Marsh Melissa Apodaca Dr. A. Wijte CSULB

Introduction Characteristics of tidal salt marshes Variable conditions Feeding and nursery grounds Sedimentary microalgae Diatoms, cyanobacteria, and filamentous green algae Important in food webs, nutrient cycling, and as sediment stabilizers

Introduction Natural and constructed marshes Previous studies Sediment characteristics Nutrient availability Previous studies Focused on primary production Sedimentary microalgae and oxygen production

Goals of the Study Determine the standing stock of sedimentary microalgae and their rates of oxygen production in a natural and constructed salt marsh Hypotheses Areas with a higher chlorophyll content will have a higher rate of oxygen production The constructed marsh will have a lower sedimentary algae biomass than the natural

Study Site Constructed marsh in San Pedro Natural marsh in Seal Beach Length of sampling Sampling habitats Canopy and exposed sites

Study Site cont.

Study Site cont. 2000 by Lisa L. Ingram

Overview of Methods Transects in intertidal Sediment cores 8cm diameter, 5cm height Chlorophyll a extraction n=10 Oxygen production measurements

Sediment Chlorophyll a Content Chlorophyll a extraction Performed on top 5mm Extraction with DMF (N,N-dimethyl formamide) at 4ºC overnight Absorbency readings at 664nm Chlorophyll a determination

Oxygen Production Measurements Oxygen readings performed on top 5mm Incubation chamber Clear plastic container covered by a glass funnel Incubation for 1 hour under light conditions normally experienced Determination of oxygen production

Statistical Analysis Chlorophyll Content and Oxygen Production Two-way ANOVA (α=0.05) H0: μ1=μ2 Effect of chlorophyll content on oxygen production Regression Analysis (α=0.05) H0: β=0

Expected Summer Results

Expected Summer Results cont.

Expected Results cont.

Expected Long Term Results

Conclusions Relationship between chlorophyll content and oxygen production Constructed vs natural marsh Importance of oxygen production by sedimentary microalgae

Future Studies Comparison of chlorophyll and oxygen production for multiple natural marshes Comparison of water column and sediment oxygen production

Acknowledgements Dr. A Wijte Siggi von Gesjen HHMI