Dihybrid cross Used when looking at inheritance patterns of 2 genes on different chromosomes Independent assortment will separate the 2 homologous chromosomes independently from each other Because of this, you must find the probability that each allele on each chromosome will combine with the other alleles
How? Lets cross 2 hybrid tall hybrid green peas with each other. Determine the genotype of these seeds
TtGg x TtGg
TtGg x TtGg The “T” has an equal chance of combining with the G or g Likewise the “t” has an equal chance Therefore we must show this in the Punnett square
TtGg x TtGg TtGg TG
TtGg x TtGg TtGg TG Tg
TtGg x TtGg TtGg TG Tg tG
TtGg x TtGg TtGg TG Tg tG tg
TtGg x TtGg TG Tg tG tg Do the same for the other pair of chromosomes down the side TG Tg tG tg
TtGg x TtGg Fill in the squares as before TG Tg tG tg TG TTGG Tg tG tg
TtGg x TtGg Determine phenotypes TG Tg tG tg TG TTGG TTGg TtGG TtGg Tg
TG Tg tG tg TG TTGG TTGg TtGG TtGg Tg TTGg TTgg TtGg Ttgg tG TtGG TtGg ttGG ttGg tg TtGg Ttgg ttGg ttgg T?G? = tall green T?gg = tall yellow ttG? = short green ttgg = short yellow
TG Tg tG tg TG TTGG TTGg TtGG TtGg Tg TTGg TTgg TtGg Ttgg tG TtGG TtGg ttGG ttGg tg TtGg Ttgg ttGg ttgg T?G? = tall green 9 T?gg = tall yellow 3 ttG? = short green 3 ttgg = short yellow 1
Cross a hybrid tall hybrid green pea plant with a short yellow plant
Cross a hybrid tall hybrid green pea plant with a short yellow plant TtGg x ttgg
Cross a hybrid tall hybrid green pea plant with a short yellow plant TtGg x ttgg TG Tg tG tg tg tg tg tg
Cross a hybrid tall hybrid green pea plant with a short yellow plant TtGg x ttgg TG Tg tG tg TtGg Ttgg ttGg ttgg tg TtGg Ttgg ttGg ttgg tg tg TtGg Ttgg ttGg ttgg tg TtGg Ttgg ttGg ttgg
TG Tg tG tg TtGg Ttgg ttGg ttgg tg TtGg Ttgg ttGg ttgg tg tg TtGg Ttgg ttGg ttgg tg TtGg Ttgg ttGg ttgg T?G? = tall green T?gg = tall yellow ttG? = short green ttgg = short yellow
TG Tg tG tg TtGg Ttgg ttGg ttgg tg TtGg Ttgg ttGg ttgg tg tg TtGg Ttgg ttGg ttgg tg TtGg Ttgg ttGg ttgg T?G? = tall green 1 T?gg = tall yellow 1 ttG? = short green 1 ttgg = short yellow 1