Biocidal Activity of Natural Euphorbia Pancorbo, S. & Hammer R. (1972). Preliminary phytochemical investigation of Euphorbia Milii. Journal Pharmaceutical Sciences, volume 6(issue 6)page 954-957. Retrieved from: onlinelibrary.wiley.com/doi/10.10021/jps.2600610631/abstract Basma, A.A. & Zakaria, Z. (2011). Antioxidant activity and phytochemical screening of the Methanol extracts of Euphorbia Hirta L. Asian Pacific Journal of Tropical Medicine. Vol. 4Issue 5. Retrieved from: www.apjtm.net/admin/picture/uploadfile/201122991223686pdf Biocidal Activity of Natural Euphorbia Milii Dye on Cotton Textiles Gianne Erika A. Romero Raja Soliman Science and Technology High School Methodology (MCB assay) Leaves of E. Milii was extracted by purifying and submerging it to acetone for 5 days. The anti-bacterial susceptibility test was conducted by letting bacteria culture multiply in 24 hours in an agar plate. Nutrient agar was inoculated to the sample by swabbing its surface. NA plates were incubated and observed for 24 hours. The clearing zone and the average diameter of the clearing zone was calculated Methodology (Natural Dye Color Fastness) The sample fabrics, one dyed and one undyed, were sewn together for better comparison. They were treated from a water shaking bath for 30 minutes and was set aside until dry. The side of the fabric tested was exposed to Xenon lamp under controlled conditions for 1 hour. The side of fabric tested was rubbed with pieces of standard wool felt under hand pressure with back and forth motion. All 3 test’s end products assessed color change with green scale comparison. Introduction Euphorbia -the largest genus of medicinal plants distributed amongst a large part of central Asia with molluscicidal, anti-microbial and anti-bacterial properties. Natural Dyes -play a huge part on the Philippine Textile Industry because of it’s synthetic and natural utilization of dyes from vast genus of plants available in the Philippines. Problem -Tinea Pinedis continuously spreads because of lack of products to help mitigate the disease. Natural dyes are also becoming scarce because of plant extinction. Objective -To promote potent cure to Athlete’s foot and other skin diseases and to present a wider variety of natural plant dyes. Leaves were minced and submerged in acetone to extract color The microbiological suspension underwent anti-microbial susceptibility test Microbial suspension was prepared from 24-hour old culture of Staphylococcus Aureus Nutrient agar plates were inoculated with the microbial suspension by swabbing the agar surface NA plates were incubated and observed for 24 hours The clearing zone and the average diameter of the clearing zone was calculated COLOR FASTNESS TEST Wash Fastness Light Fastness Rub fastness Prepare the dyed clothe and the stock solution and wash manually then let it dry. Green Scale Assessment Expose test specimen to Xenon lamp for 1 hour. Apply hand pressure in synchronized motion. Tools Submerged leaves Results and Discussion The sample of E. Milii dye did not inhibit the growth of S. Aureus because of careless purification and distillation. Therefore, lacks support in having potential anti-microbial properties. However, it showed good results in color fastness to be a natural dye without mixing chemical compounds. TEST ORGANISM Sample Clearing zone mm AI 1 2 3 S. Aureus Euphorbia Milii Dye -ª - Ofloxacin discᵇ 33 4.5 Euphorbia Milii Leaves Acetone Submerged Cotton Cloth Fastness grades according to grey scales Mordanting method Mordant Proportions Light fastness Wash Fastness Rub Fastness Pre-mordanting 3:1 1:2 1:3 4 2-3 4-5 5 Simultaneous Mordanting Post mordanting End product Dyed Cloth and Dye Sample Summary and Conclusion This research studied the potential of E. Milii dye to have anti-microbial activity and it’s ability to sustain color when applied in cotton textiles. This study was needed an anti-microbial assay of the sample subjected to the gram negative bacteria. This study also conducted color fastness test which is based on the following components: Light fastness, Rub fastness and Light fastness. Based on the results, it can be obtained that E. Milii dye can be an effective anti-microbial product if given with right treatment and experimentation. It also is an effective natural, non-synthetic dye that needs no chemical to have excellent color fastness properties. This research achieved the goal of producing novel sources of dyes. Literature Cited Pancorbo, S. & Hammer R. (1972). Preliminary phytochemical investigation of Euphorbia Milii. Journal Pharmaceutical Sciences, volume 6(issue 6)page 954-957. Retrieved from: onlinelibrary.wiley.com/doi/10.10021/jps.2600610631/abstract   Basma, A.A. & Zakaria, Z. (2011). Antioxidant activity and phytochemical screening of the Methanol extracts of Euphorbia Hirta L. Asian Pacific Journal of Tropical Medicine. Vol. 4Issue 5. Retrieved from: www.apjtm.net/admin/picture/uploadfile/201122991223686pdf