Introduction Conclusions

Slides:

Advertisements

Similar presentations

Isosteviol derivatives induced apoptosis in Human lung cancer via targeting MEK/MAPK pathway: An in vitro and in vivo study Ahmed M Malki 1,,PhD Stephen.

Advertisements

第三章 Survivin siRNA nano particles are capable of inhibiting liver cancer cell growth both in vitro and in vivo Suoqin Tang,MD, Kuiyao Qu,MD, Yi Zhang,MD.

Cell Physiol Biochem 2017;42:2582– DOI: /

In Vitro and In Vivo Studies of Non-Platinum-Based Halogenated Compounds as Potent Antitumor Agents for Natural Targeted Chemotherapy of Cancers Qing-Bin.

Enhanced Sensitivity to Sunitinib by Inhibition of Akt1 Expression in Human Castration- resistant Prostate Cancer PC3 Cells Both In Vitro and In Vivo

A Novel Cinnamide YLT26 Induces Breast Cancer Cells Apoptosis via ROS-Mitochondrial Apoptotic Pathway in Vitro and Inhibits.

Corresponding author:

Aldehyde Dehydrogenase 1A1 Possesses Stem-Like Properties and Predicts Lung Cancer Patient Outcome Xiao Li, MD, Liyan Wan, MD, Jian Geng, MD, Chin-Lee.

Introduction Conclusions

Saikosaponin-D Enhances Radiosensitivity of Hepatoma Cells under Hypoxic Conditions by Inhibiting Hypoxia-Inducible Factor-1α Cell Physiol Biochem 2014;33:37-51.

Figure 1. Herbacetin binds to AKT1/2 and suppresses each respective kinase activity. The effect of herbacetin on (A) PI3K/AKT and (B) MAPK signaling pathway.

Corresponding author:

MicroRNA-101 Inhibits Growth, Proliferation and Migration and Induces Apoptosis of Breast Cancer Cells by Targeting Sex-Determining Region Y-Box 2 Cell.

Fig. 6. Knockdown of TCF-4 inhibits TCTP-induced glioma cell proliferation in vitro and in vivo. (A) TCF-4 was knocked down in U-251/TCTP and H-4/TCTP.

Inhibitory effect of (-)-epigallocatechin-3-gallate and bleomycin on human pancreatic cancer MiaPaca-2 cell growth Bimonte S1, Leongito M1, Barbieri.

Elevated FOXC2 Expression Promotes Invasion of HCC Cell Lines and is Associated with Poor Prognosis in Hepatocellular Carcinoma Cell Physiol Biochem 2017;44:99–109.

Figure 1. Herbacetin binds to AKT1/2 and suppresses each respective kinase activity. The effect of herbacetin on (A) PI3K/AKT and (B) MAPK signaling pathway.

Volume 18, Issue 12, Pages (December 2016)

Cyclin-Dependent Kinase 2 Promotes Tumor Proliferation and Induces Radio Resistance in Glioblastoma Jia Wang, Tong Yang, Gaofeng Xu, Hao Liu, Chunying.

Knockdown of Bone Morphogenetic Proteins Type 1a Receptor (BMPR1a) in Breast Cancer Cells Protects Bone from Breast Cancer-Induced Osteolysis by Suppressing.

Volume 144, Issue 3, Pages e4 (March 2013)

Vemurafenib Induces Senescence Features in Melanoma Cells

Deregulation of SLIT2-Mediated Cdc42 Activity Is Associated with Esophageal Cancer Metastasis and Poor Prognosis Ruo-Chia Tseng, PhD, Jia-Ming Chang,

Droxinostat, a Histone Deacetylase Inhibitor, Induces Apoptosis in Hepatocellular Carcinoma Cell Lines via Activation of the Mitochondrial Pathway and.

The Chemopreventive Effect of Tanacetum Polycephalum Against LA7-Induced Breast Cancer in Rats and the Apoptotic Effect of a Cytotoxic Sesquiterpene.

IFN-γ Induces Gastric Cancer Cell Proliferation and Metastasis Through Upregulation of Integrin β3-Mediated NF-κB Signaling Yuan-Hua Xu, Zheng-Li Li,

Volume 144, Issue 2, Pages (February 2013)

Intermittent cyclic mechanical tension promotes endplate cartilage degeneration via canonical Wnt signaling pathway and E-cadherin/β-catenin complex cross-talk

Hydrogen Sulfide Demonstrates Promising Antitumor Efficacy in Gastric Carcinoma by Targeting MGAT5 Rui Wang, Qilin Fan, Junjie Zhang, Xunan Zhang, Yuqi.

NF-κBp65-specific siRNA inhibits expression of genes of COX-2, NOS-2 and MMP-9 in rat IL-1β-induced and TNF-α-induced chondrocytes Dr C. Lianxu, Ph.D.,

Cell Physiol Biochem 2016;39: DOI: /

Volume 9, Issue 2, Pages (August 2017)

The Zinc Ionophore PCI-5002 Radiosensitizes Non-small Cell Lung Cancer Cells by Enhancing Autophagic Cell Death Kwang Woon Kim, PhD, Christina K. Speirs,

Aldehyde Dehydrogenase 1A1 Possesses Stem-Like Properties and Predicts Lung Cancer Patient Outcome Xiao Li, MD, Liyan Wan, MD, Jian Geng, MD, Chin-Lee.

Antisense Oligonucleotides Targeting Y-Box Binding Protein-1 Inhibit Tumor Angiogenesis by Downregulating Bcl-xL-VEGFR2/-Tie Axes Kiyoko Setoguchi, Lin.

Glyoxalase I Is Differentially Expressed in Cutaneous Neoplasms and Contributes to the Progression of Squamous Cell Carcinoma Xiao-Yan Zou, Dong Ding,

Volume 6, Issue 1, Pages (January 2014)

Toll-Like Receptor 5 Engagement Modulates Tumor Development and Growth in a Mouse Xenograft Model of Human Colon Cancer Sang Hoon Rhee, Eunok Im, Charalabos.

Transdifferentiation of Melanoma Cells by the Reprogramming Factors Attenuates Malignant Nature In Vitro and In Vivo Mikiro Takaishi, Shigetoshi Sano

Uc.454 Inhibited Growth by Targeting Heat Shock Protein Family A Member 12B in Non- Small-Cell Lung Cancer Jun Zhou, Chenghai Wang, Weijuan Gong, Yandan.

Volume 118, Issue 3, Pages (August 2004)

Volume 141, Issue 6, Pages e5 (December 2011)

Volume 25, Issue 3, Pages (March 2017)

NF-κB Inhibition through Proteasome Inhibition or IKKβ Blockade Increases the Susceptibility of Melanoma Cells to Cytostatic Treatment through Distinct.

Volume 142, Issue 7, Pages e2 (June 2012)

Molecular Therapy - Nucleic Acids

Inhibition of KLF4 by Statins Reverses Adriamycin-Induced Metastasis and Cancer Stemness in Osteosarcoma Cells Yangling Li, Miao Xian, Bo Yang, Meidan.

Heather L Maecker, Zhong Yun, Holden T Maecker, Amato J Giaccia

Volume 23, Issue 1, Pages (January 2015)

Ribosomal Protein S3 Gene Silencing Protects Against Cigarette Smoke-Induced Acute Lung Injury Jinrui Dong, Wupeng Liao, Hong Yong Peh, W.S. Daniel Tan,

miR-124 Inhibits Lung Tumorigenesis Induced by K-ras Mutation and NNK

Volume 6, Issue 1, Pages (January 2014)

Molecular Therapy - Nucleic Acids

Myeloma cell–derived Runx2 promotes myeloma progression in bone

Select Cancer Testes Antigens of the MAGE-A, -B, and -C Families Are Expressed in Mast Cell Lines and Promote Cell Viability In Vitro and In Vivo Bing.

Lu Zheng, Nan You, Xiaobing Huang, Huiying Gu, Ke Wu, Na Mi, Jing Li

Molecular Therapy - Nucleic Acids

Volume 19, Issue 8, Pages (August 2011)

Suppression of IGF1R in Melanoma Cells by an Adenovirus-Mediated One-Step Knockdown System Haoran Xin, Mingxing Lei, Zhihui Zhang, Jie Li, Hao Zhang,

Figure 1. RSPO3 expression is upregulated in bladder cancer

Figure 1. LOC is highly expressed in NPC and predicts unfavorable prognosis. (A) Differential gene expression ... Figure 1. LOC is highly expressed.

Volume 17, Issue 2, Pages (February 2009)

Green Tea Polyphenol Epigallocatechin-3-Gallate Suppresses Collagen Production and Proliferation in Keloid Fibroblasts via Inhibition of the STAT3-Signaling.

Shrimp miR-34 from Shrimp Stress Response to Virus Infection Suppresses Tumorigenesis of Breast Cancer Yalei Cui, Xiaoyuan Yang, Xiaobo Zhang Molecular.

Syed M. Meeran, Thejass Punathil, Santosh K. Katiyar

Long Noncoding RNA BC as a Novel Therapeutic Target for Colorectal Cancer that Suppresses Metastasis by Upregulating TIMP3 Jiaxin Lin, Xin Tan,

Molecular Therapy - Nucleic Acids

Fig. 6 RUNX/CBFB interaction inhibitor, Ro5-3335, significantly decreases mouse neurofibroma growth in vivo. RUNX/CBFB interaction inhibitor, Ro5-3335,

Paracrine Apoptotic Effect of p53 Mediated by Tumor Suppressor Par-4

Antitumor effects of celastrol in vitro and in vivo.

Presentation transcript:

Introduction Conclusions Curcumin inhibits tumor growth and angiogenesis in an orthotopic mouse model of human pancreatic cancer Bimonte S.1; Barbieri A.2; Palma G.2; Rea D.2; Luciano A2., Arra C.2 and Izzo F.1 1 Hepatobiliary Unit, Istituto Nazionale per lo studio e la cura dei Tumori "Fondazione G. Pascale", - IRCCS-ITALIA Via Mariano Semmola, 80131, Naples, Italy; 2 INT Facility, Istituto Nazionale per lo studio e la cura dei Tumori "Fondazione G. Pascale", - IRCCS-ITALIA Via Mariano Semmola, 80131, Naples, Italy Corresponding Author: Sabrina Bimonte mail:s.bimonte@istitutotumori.na.it Introduction Pancreatic cancer is a malignant neoplasm originating from transformed cells arising in tissues forming the pancreas. The best chemotherapeutic agent used to treat pancreatic cancer is the gemcitabine. However, gemcitabine treatment is associated with many side effects. Thus novel strategies involving less toxic agents for treatment of pancreatic cancer are necessary. Curcumin is one such agent that inhibits the proliferation and angiogenesis of a wide variety of tumor cells, through the modulation of many cell signalling pathways. In this study, we investigated whether curcumin plays antitumor effects in MIA PaCa-2 cells. In vitro studies showed that curcumin inhibits the proliferation and enhances apoptosis of MIA PaCa-2 cells. To test whether the antitumor activity of curcumin is also observed in vivo, we generated an orthotopic mouse model of pancreatic cancer by injection of MIA PaCa-2 cells in nude mice. We placed mice on diet containing curcumin at 0.6% for 6 weeks. In these treated mice tumors were smaller with respect to controls and showed a downregulation of the transcription nuclear factor NF-κB and NF-κB-regulated gene products. Overall, our data indicate that curcumin has a great potential in treatment of human pancreatic cancer through the modulation of NF-κB pathway. Curcumin inhibits the tumor growth in orthotopic mouse model of pancreatic cancer. (A) MacroFluo images of fluorescent analysis tumor area images control and treated mice. (B) Measurements of fluorescence per second depicting tumor volume at different time points using Macrofluo images, showed that the final tumor volumes on day 35 after the start of treatment is significantly decreased in the curcumin group compared with control (P = 0.00393). Curcumin inhibits proliferation and enhances apoptosis in MIA PaCa-2 cells. (a)–(f) Wound assay results show suppression of MIA PaCa-2 cell proliferation induced by curcumin at 48 h (b) With respect to controls. The results are the mean of total invaded areas of three distinct sections on the slide per 48 h. (P = 0.02, Mann-Whitney U test). (g) MTT assay results show a suppression of proliferation in pancreatic cancer cells treated with curcumin respect to control cells. Data are representative of two independent experiments (P value <0.05). (h) In vitro apoptosis assay by flow cytometry indicated that curcumin enhances apoptosis in MIA PaCa-2 cells. (i) EMSA results showes that curcumin suppresses activation of NF-κB in MIA PACA-2 cells. (j) In vitro apoptosis assay by flow cytometry indicates that silencing of NF-κB using siRNA κB is not able to potentiate the apoptotic effects of curcumin on MIA PaCa-2 cells. Curcumin inhibits NF-κB activation and downregulates NF-κB-regulated gene products in orthotopic pancreatic tumors. (A) EMSA assay performed on orthotopic tumor tissue samples showed the inhibition of NF-κB by curcumin. (B) Immunohistochemical analysis for nuclear COX-2 and VEGF showed the inhibition of COX-2 and VEGF expression in curcumin-treated group, compared to controls (Figure 3B), lower panels). Percentages indicate the positive staining for the given biomarker. (C) Western blot showing that curcumin inhibits the expression of NF-κB-dependent gene products VEGF, cyclin D1, MMP-9, COX-2, IKKα, and IKKβ in pancreatic tumor tissues. Samples from three animals in each group were analysed, and representative data are shown. β-Actin was used as loading control. Conclusions Curcumin has a great potential in treatment of human pancreatic cancer through the modulation of NF-κB pathway.