Presenting author: maheshmg2012@gmail.com, *yash28bhandary@gmail.com Expression levels of Inflammatory cytokines and p53-fibrinolytic system in smokers with or without COPD Mahesh MG1, Deepu C2, Irfan KM2, Ashwini S3, Bhandary YP1* Yenepoya Research Centre, Yenepoya University, Deralakatte, Mangalore-575018 Presenting author: maheshmg2012@gmail.com, *yash28bhandary@gmail.com Results Your text would go here. INTRODUCTION RESULTS Samples Control Smokers with COPD Smokers without COPD Blood 3 9 8 Serum Sputum Chronic obstructive pulmonary disease (COPD) is a disease characterized by inflammation in airway and obstruction of lung function. COPD patient are more often found with alveolar inflammation due to accumulation of inflammatory cells, elevated pulmonary IL-17A. Defective fibrinolysis and alveolar fibrin deposition due to a disproportionate increase in plasminogen activator inhibitor (PAI)-1 and epithelial cell apoptosis. Figure 1: IL-17A expression levels in normal and COPD patient. Blood samples were collected from normal and COPD patients. The serum samples were subjected to western blotting analysis for IL-17A using IL-17 A antibody. Samples were normalized with β-actin antibody. Table-1: Collection of Blood and sputum samples collected. Parameters Smokers with COPD Stage I Stage II Stage III Stage IV No of Patients 9 4 3 2 - Average Age 40-85 40-60 55-85 50-70 Sex M Smokers without COPD 8 30-70 Normal control No. 40-65 Figure 2: p53 and P-p53 expression levels in normal and COPD patient. Blood samples were collected from normal and COPD patients. The serum samples were subjected to western blotting analysis for p53, and PAI-1 expression levels using their respective antibody. Samples were normalized with β -actin antibody. Table-3: Categorization of COPD samples based on FEV1/FVC ratio. WORK FLOW Normal Smokers with COPD Smokers without COPD Stage I Stage II Stage III Stage IV  Eosinophils  Hb  MCH  Lymphocytes Lymphocytes  PCV - Collection of blood samples Biochemical analysis Screening for IL-17A expression Screening for p53-fibrinolytic system expression Figure 3: Cleaved and total caspase-3 levels in normal and COPD patient. Blood samples were collected from normal and COPD patients. The serum samples were subjected to western blotting analysis for cl.caspase-3 and t.caspase-3 levels using their respective antibody. Samples were normalized with β-actin antibody Table-2: Biochemical analysis of COPD samples. DISCUSSION No major changes in the biochemical analysis seen among smokers with or without COPD Increased expression of IL-17A and p53-fibrinolytic system was observed in smokers with COPD The elevated expression of IL-17A plays a major role in p53 expression and its regulation CONCLUSION A novel approach to prove the IL-17A mediated p53-fibrinolytic system during COPD. It may be useful to find out a novel pathway for the better understanding of molecular mechanism involved in COPD. ACKNOWLEDGEMENT The authors acknowledge Yenepoya Research Centre, and Department of pulmonary medicine, Yenepoya Medical college, Yenepoya University for the support