Volume 101, Issue 4, Pages 487-498 (April 2006) Ultrastructural changes of the palatal mucosa following application of 4-nitroquinoline-l- oxide (4NQO) in rats subjected to major salivary gland excision Antonia Sioga, MD, PhD, Louise Economou, MD, PhD, Eleftherios G. Kaklamanos, DDS, Vasilios Antoniades, DDS, George Keramidas, MD, Anastasios Manthos, MD, PhD, Kostantinos Antoniades, MD, DDS, PhD Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontics Volume 101, Issue 4, Pages 487-498 (April 2006) DOI: 10.1016/j.tripleo.2005.05.067 Copyright © 2006 Mosby, Inc. Terms and Conditions
Fig. 1 Group A. 13 weeks. Basal layers of epithelium with increased intercellular spaces () and reduction of hemidesmosomes () (×8000). Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontics 2006 101, 487-498DOI: (10.1016/j.tripleo.2005.05.067) Copyright © 2006 Mosby, Inc. Terms and Conditions
Fig. 2 Group A. 16 weeks. Basal cytoplasmic processes (). N: nucleus of the cuboidal basal cells (×5000). Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontics 2006 101, 487-498DOI: (10.1016/j.tripleo.2005.05.067) Copyright © 2006 Mosby, Inc. Terms and Conditions
Fig. 3 Group A. 16 weeks. Accumulation of desmosomes (d) between the cells of middle layers, bundle of tonofilaments (f) in the cytoplasm (×16 000). Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontics 2006 101, 487-498DOI: (10.1016/j.tripleo.2005.05.067) Copyright © 2006 Mosby, Inc. Terms and Conditions
Fig. 4 Group A. 23 weeks. Accumulation of damaged desmosomes (d), bundles of tonofilaments (f) in cells of middle layers, with high nucleocytoplasmic index (N) (×8000). Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontics 2006 101, 487-498DOI: (10.1016/j.tripleo.2005.05.067) Copyright © 2006 Mosby, Inc. Terms and Conditions
Fig. 5 Group A. 23 weeks. The basement membrane is discontinuous (), the basal cytoplasmic processes are very large (), there are a lot of tonofilaments (f) in middle and basal cells, with high nucleocytoplasmic index and well-developed nucleolus (n). c, capillary in the connective tissue. A grey line has been drawn to emphasize the discontinuity of the basement membrane (×6000). Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontics 2006 101, 487-498DOI: (10.1016/j.tripleo.2005.05.067) Copyright © 2006 Mosby, Inc. Terms and Conditions
Fig. 6 Group B. 13 weeks. Epithelium (E) with many layers of flat cells, basement membrane (), connective tissue (ct) (×4000). Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontics 2006 101, 487-498DOI: (10.1016/j.tripleo.2005.05.067) Copyright © 2006 Mosby, Inc. Terms and Conditions
Fig. 7 Group B. 13 weeks. The basal cells have many bundles of tonofilaments (f), high nucleocytoplasmic index, and large nucleus with well-developed nucleolus (n). The basement membrane is discontinuous (). A grey line has been drawn to emphasize the discontinuity of the basement membrane (×6000). Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontics 2006 101, 487-498DOI: (10.1016/j.tripleo.2005.05.067) Copyright © 2006 Mosby, Inc. Terms and Conditions
Fig. 8 Group B. 10 weeks. Increased intercellular spaces () in the middle layers of epithelium (×4000). Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontics 2006 101, 487-498DOI: (10.1016/j.tripleo.2005.05.067) Copyright © 2006 Mosby, Inc. Terms and Conditions
Fig. 9 Group B. 20 weeks. Large basal cytoplasmic processes (), discontinuous basement membrane (). A grey line has been drawn to emphasize the discontinuity of the basement membrane (×10 000). Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontics 2006 101, 487-498DOI: (10.1016/j.tripleo.2005.05.067) Copyright © 2006 Mosby, Inc. Terms and Conditions
Fig. 10 Group B. 16 weeks. The cells are separated, with pleomorphic nucleus (N) and mitotic figures (M), discontinuous basement membrane (). A grey line has been drawn to emphasize the discontinuity of the basement membrane (×4000). Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontics 2006 101, 487-498DOI: (10.1016/j.tripleo.2005.05.067) Copyright © 2006 Mosby, Inc. Terms and Conditions
Fig. 11 Group B. 25 weeks. Large nuclei with prominent multiple nucleoli (n), pyknotic nuclei (N) (×4000). Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontics 2006 101, 487-498DOI: (10.1016/j.tripleo.2005.05.067) Copyright © 2006 Mosby, Inc. Terms and Conditions
Fig. 12 Group B. 23 weeks. The basement membrane is discontinuous () in a large part of epithelium (×20 000). Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontics 2006 101, 487-498DOI: (10.1016/j.tripleo.2005.05.067) Copyright © 2006 Mosby, Inc. Terms and Conditions
Fig. 13 Group C. Animals with major salivary glands. Middle layer cells. N: nuclei. (×4000). Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontics 2006 101, 487-498DOI: (10.1016/j.tripleo.2005.05.067) Copyright © 2006 Mosby, Inc. Terms and Conditions
Fig. 14 Group C. Animals subjected to major salivary glands excision. Middle layer cells. : lightly dilated intercellular spaces (×4000). Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontics 2006 101, 487-498DOI: (10.1016/j.tripleo.2005.05.067) Copyright © 2006 Mosby, Inc. Terms and Conditions
Fig. 15 Group C. Animals subjected to major salivary glands excision. Keratin layer. (×4000). Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontics 2006 101, 487-498DOI: (10.1016/j.tripleo.2005.05.067) Copyright © 2006 Mosby, Inc. Terms and Conditions