Copyright © 2010 American Medical Association. All rights reserved. From: Improvement of Visual Performance With Intravitreal Administration of 9-cis-Retinal in Rpe65-Mutant Dogs Arch Ophthalmol. 2010;128(11):1442-1448. doi:10.1001/archophthalmol.2010.210 Figure Legend: Result of the second 9-cis-retinal injection in the same eye for dogs 5 and 6. The mean (SD) a-wave (A) and b-wave (B) dark-adapted intensity response amplitudes are shown. In each graph, the solid line is the mean result from the first 9-cis-retinal injection, the long dashed line is the mean result from the second 9-cis-retinal injection, and the short dashed lines are the mean results from the vehicle-injected eyes. The second injection was performed 29 weeks after the first for dog 5 and 20 weeks later for dog 6. Electroretinograms before the second injection showed that response had returned to baseline following initial rescue by the first injection. Date of download: 10/3/2017 Copyright © 2010 American Medical Association. All rights reserved.

Copyright © 2010 American Medical Association. All rights reserved. From: Improvement of Visual Performance With Intravitreal Administration of 9-cis-Retinal in Rpe65-Mutant Dogs Arch Ophthalmol. 2010;128(11):1442-1448. doi:10.1001/archophthalmol.2010.210 Figure Legend: Comparison of electroretinogram responses from pretreatment and then 9-cis-retinal–injected and vehicle-injected eyes of an Rpe65−/− dog (dog 1). A, Dark-adapted and light-adapted electroretinogram intensity response series from pretreatment, 1 week after treatment, and 4 weeks after treatment. The right eye was injected with 9-cis-retinal and the left eye received vehicle. The vertical line across the tracings indicates the timing of the flash. Vertical size bar for dark-adapted series = 50 μV; for light-adapted series = 20 μV. Horizontal bars = 50 milliseconds. Dark-adapted flash intensities range from −2.79 to 2.4 log cdS/m2. Light-adapted flash intensities range from −0.2 to 2.8 log cdS/m2. B, Rod (dark-adapted 5-Hz series, −1.6 log cdS/m2) and cone (light-adapted to 30 cd/m2 33-Hz series, 0.39 log cdS/m2) flicker preinjection and 1 and 4 weeks after injection. The upper tracings are from the right eye (9-cis-retinal treated) and the lower tracings, from the left eye (vehicle injected). Vertical bars are 5 μV. Horizontal bars are 500 milliseconds for 5-Hz flicker and 50 milliseconds for 33-Hz flicker. Dashed vertical lines indicate timing of flashes. Date of download: 10/3/2017 Copyright © 2010 American Medical Association. All rights reserved.

Copyright © 2010 American Medical Association. All rights reserved. From: Improvement of Visual Performance With Intravitreal Administration of 9-cis-Retinal in Rpe65-Mutant Dogs Arch Ophthalmol. 2010;128(11):1442-1448. doi:10.1001/archophthalmol.2010.210 Figure Legend: Dark-adapted mean a- and b-wave electroretinogram intensity response plots (log:log scale) for normal dogs of similar breeding (n = 9), the treated dogs with rescue (n = 5), and the vehicle-injected eyes of the same dogs (n = 5). Error bars are +1 SD. The mean a-wave (A) and mean b-wave (B) amplitudes for the treated eyes are significantly greater than those of the vehicle-injected eyes at each flash intensity (P < .05). Date of download: 10/3/2017 Copyright © 2010 American Medical Association. All rights reserved.