Fig. 6 Gene expression and DNA methylation in callus

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Fig. 6 Gene expression and DNA methylation in callus Fig. 6 Gene expression and DNA methylation in callus. (a) Relative transcript level of HsfA2 in seedlings and callus before the heat stress (NS), immediately after the heat stress (HS) and 2 weeks after the heat stress (HS + 2 wk). (b) Relative transcript level of ONSEN in seedlings and callus before the heat stress (NS), immediately after the heat stress (HS) and 2 weeks after the heat stress (HS + 2 wk). Asterisks mark significant differences compared with wild-type seedlings (P < 0.05). (c) Methylation analysis of ONSEN by DNA gel blotting of NS and HS callus. Arrowheads indicate the extrachromosomal DNA of ONSEN. A gel stained with ethidium bromide (EtBr) was used as a loading control. From: Inducible Transposition of a Heat-Activated Retrotransposon in Tissue Culture Plant Cell Physiol. 2016;58(2):375-384. doi:10.1093/pcp/pcw202 Plant Cell Physiol | © The Author 2016. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com 1