Bacterial expression SystemBacterial expression System in Creative BioMart

Over the years, Creative BioMart has developed BacTEC TM and FoldEZ TM technologies to tackle hard- to-express and hard-to-dissolve proteins. With BacTEC TM technology, the protein expression level can be greatly improved for almost all of the proteins. In addition, over 98% of the inclusion bodies can be refolded and solubilized by our proprietary FoldEZ TM protein refolding technology.protein expression

Slide Title Typically, the first choice for the expression of recombinant proteins is E. coli. Protein product in E. coli expression is fast, convinent, well- established and usually high yields.E. coli expression Newly-built facilities supporting a vast array of state-of-the-art equipment are leveraged by our experienced PhD-level scientists to deliver proteins expressed via microbial systems quickly and economically.

“One of the most popular technologies is BacTEC TM system. Creative BioMart's patented BacTEC TM system is capable of achieving high levels of soluble expression proteins. While the technology has shown it can express many types of proteins, it has established a track record for producing high-quality, correctly folded antibody fragments (including Fabs and scFv).”

Our BacTEC TM protein refolding technology is designed to determine the optimal refolding conditions by evaluation a series of buffers. We provide refolded protein at purity of 95% or greater. Exclusively from Creative BioMart, OxiCyto TM competent E. coli strains are engineered to enable the folding of recombinantly expressed proteins in vivo. The expression of the isomerase, DsbC, within the OxiCyto cytoplasm enhances the correct formation of disulfide bonds in proteins that require them for biological activity.

Our Bacillus subtilis system is an ideal option for the expression of monomeric protein products at high expression levels. This high yield, combined with our patented fermentation protocols allow for fast process development and scale-up to support early clinical testing.

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