Selective and Differential Media

Slides:

Advertisements

Similar presentations

Blood Cells Antibiotic Sensitivity. Neutrophil (nucleus has several lobes) Present in high numbers during bacterial infection.

Advertisements

EMB agar Only gram-negative bacteria grow on EMB agar. (Gram-positive bacteria are inhibited by the dyes eosin and methylene blue added to the agar.) Based.

Differential medium. Differential medium helps us to differentiate one group of bacteria from another. Blood agar – differentiate hemolytic bacteria from.

Ex. 14: Skin Cultures and Importance of Selective and Differential Media for Isolating Gram-Positive Cocci Objectives??

Staphylococcus Gram + cocci In clumps.

A B C D E F G H I J L K Infectious Diseases Unit 2 Lesson 7 plan.

Isolation and Identification of Staphylococci

Micro labs - review BIOL260 Winter Ubiquity What organisms grow best at room temperature? ___°C? At body temperature? = ___°C? What kind of medium.

Lab 14 Goals and Objectives: Exercise 69: Staphylococci Identification Read and record results Exercise 70: Streptococci & Enterococci Identification Read.

Introduction In addition to general-purpose media, which allow the growth of most types of bacteria, microbiologists use specialized media to identify.

Selective and Differential Media

Ph. D Abdelraouf A. Elmanama

Microbial Growth Chapter 6.

Media Preparation & Sterilization

Selective and Differential media

Selective media Lab 6.

Staph and Strep.

Review Lab Exercises Exercise 8 zWhat is the purpose of this exercise? zWhat is a capsule? zWhat is the capsule made of? zWhat is the importance.

Review of Gram Stain Selective and differential Media

Selected and Differential Media. Define Selective Media Differential Media Enriched Media.

SELECTIVE, DIFFERENTIAL AND ENRICHED MEDIA

Identification of Strep and Staph and Isolating gram – pathogens Lab # 10 Medgar Evers College Prof. Santos.

2 pt 3 pt 4 pt 5pt 1 pt 2 pt 3 pt 4 pt 5 pt 1 pt 2pt 3 pt 4pt 5 pt 1pt 2pt 3 pt 4 pt 5 pt 1 pt 2 pt 3 pt 4pt 5 pt 1pt Bacterial Counts Oxygen and Temperature.

Identification of Pathogenic Bacteria by Laboratory Methods M. Kent Froberg, MD.

Lab 13- Bacterial cultivation

Staphylococcus Identification Tests

Differential and Selective Media Prepared BY Ms. c: Abed Al Rahman I

Ex. 13: Urine Culture Technique and the Importance of Selective and Differential Media for Gram-Negative Rods Objectives ??

Microbial Control Lab 4. Selective and Differential Media We have completed Isolation of bacteria using steak plate and spread plate This is a good beginning,

Identification of Staphylococci and Streptococci

Gram Positive Staphylococci – (Note the purple, spherically- shaped, clustered cells)

PHT 313 Lab (1) Staphylococci.

STAPHYLOCICCI Lecture # 3. Staphylococcus sp.  Morphology:  Gram positive cocci.  In clusters  Culture:  Facultative anaerobes  Incubation 37ºC.

STAPHYLOCICCI Basmah almaarik

Isolation of Staphylococcus aureus and Streptococcus

Selective and Differential Media. EMB Selective for Gram negative E.coli has green metallic sheen No growth of Gram pos Staph aureus.

Components Preparation Inoculation

226 PHT Final Spots. Gram’s stain Results: Shape: Cocci Arrangment: irregular clusters Colour: Violet Gram’s reaction: Gram’s +ve Name of microorganism:

General Microbiology Laboratory Isolation and Identification of Gram Positive Cocci.

Selective and differential Media By : Mahmoud W. El-Hindi

Lab 14 Goals and Objectives: Exercise 69: Staphylococci Identification Read and record results Exercise 70: Streptococci & Enterococci Identification Read.

culturing of microorganism

Urinary Tract Infection Department of Microbiology

Microbiology lab Urinary system. Urine culture steps 1.Collect the sample in sterile container: 1.Midstream catch. 2.Through a catheter. 3.Suprapubic.

Exercise 39: Oxidation and Fermentation Tests (Catalase)

Mystery Growth Laboratory Practical

Staphylococcus.

Exercise 38: Cultural Characteristics (Gelatin) put on ice!!!

Identification of Unknown Bacteria: individual assignment

Urinary Tract Infection Department of Microbiology

Culture Media م.م رنا مشعل.

Identification of Unknown Bacteria: individual assignment

Selective and Differential Media

Microbiology Lab # 11 Medgar Evers College Dr. Santos

MEDICAL MICROBIOLOGY LAB MEDI 3101

PENGENALAN KULTUR MEDIA BAKTERI

Cultivation of Bacteria

Week 5 B Exp 10: Gram Stain Gram Stain: Gram + SE, SA

Chapter 6 Microbial Growth.

Selective and differential Media By : Mahmoud W. El-Hindi

Urine Culture Technique and the Importance of Selective and Differential Media for Gram-Negative Rods Day 2 Remember this plate?

Introduction In addition to general-purpose media, which allow the growth of most types of bacteria, microbiologists use specialized media to identify.

طريقة تخطيط الاطباق Streak Plate Method

Media Preparation & Sterilization

Introduction In addition to general-purpose media, which allow the growth of most types of bacteria, microbiologists use specialized media to identify.

Presentation transcript:

Selective and Differential Media

EMB Selective for Gram negative E.coli has green metallic sheen No growth of Gram pos Staph aureus

MSA: Selective and Differential Selective because of salt: only Staph can grow Differential because S. aureus ferments mannitol sugar (turns it yellow) but Staph epidermidis does not ferment mannitol. Note there is no growth or fermentation from M. luteus.

MSA with Staph aureus

Mitis Salivarius Agar

Mitis Salivarius Agar

Mitis Salivarius Agar

BLOOD AGAR: Enrichment media, and Differential

Fish Eye Colonies of Enterobacter on blood agar

MacConkey’s Agar: Selective and Differential Selective because Gram pos are inhibited by the bile salts. Differential because it has lactose. Gram negs that ferment lactose turn yellow.

Antibiotic Sensitivity

Antibiotic Sensitivity Organism is resistant to the antibiotic Organism is susceptible to the antibiotic

Antibiotic Sensitivity: Check zone diameters on given table Organism has intermediate resistance to the antibiotic Organism is resistant to the antibiotic Organism is susceptible to the antibiotic

Coagulase Test: Differentiates S. Aureus from S epidermidis Positive: S. aureus Negative: S. epidermidis