Early allopolyploid evolution in the post-Neolithic Brassica napus oilseed genome by Boulos Chalhoub, France Denoeud, Shengyi Liu, Isobel A. P. Parkin,

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Early allopolyploid evolution in the post-Neolithic Brassica napus oilseed genome by Boulos Chalhoub, France Denoeud, Shengyi Liu, Isobel A. P. Parkin, Haibao Tang, Xiyin Wang, Julien Chiquet, Harry Belcram, Chaobo Tong, Birgit Samans, Margot Corréa, Corinne Da Silva, Jérémy Just, Cyril Falentin, Chu Shin Koh, Isabelle Le Clainche, Maria Bernard, Pascal Bento, Benjamin Noel, Karine Labadie, Adriana Alberti, Mathieu Charles, Dominique Arnaud, Hui Guo, Christian Daviaud, Salman Alamery, Kamel Jabbari, Meixia Zhao, Patrick P. Edger, Houda Chelaifa, David Tack, Gilles Lassalle, Imen Mestiri, Nicolas Schnel, Marie-Christine Le Paslier, Guangyi Fan, Victor Renault, Philipp E. Bayer, Agnieszka A. Golicz, Sahana Manoli, Tae-Ho Lee, Vinh Ha Dinh Thi, Smahane Chalabi, Qiong Hu, Chuchuan Fan, Reece Tollenaere, Yunhai Lu, Christophe Battail, Jinxiong Shen, Christine H. D. Sidebottom, Xinfa Wang, Aurélie Canaguier, Aurélie Chauveau, Aurélie Bérard, Gwenaëlle Deniot, Mei Guan, Zhongsong Liu, Fengming Sun, Yong Pyo Lim, Eric Lyons, Christopher D. Town, Ian Bancroft, Xiaowu Wang, Jinling Meng, Jianxin Ma, J. Chris Pires, Graham J. King, Dominique Brunel, Régine Delourme, Michel Renard, Jean- Marc Aury, Keith L. Adams, Jacqueline Batley, Rod J. Snowdon, Jorg Tost, David Edwards, Yongming Zhou, Wei Hua, Andrew G. Sharpe, Andrew H. Paterson, Chunyun Guan, and Patrick Wincker Science Volume 345(6199): August 22, 2014 Published by AAAS

Fig. 1 Recurrent genome duplications in B. napus.Genomic alignments between the basal angiosperm Amborella trichopoda (24), the basal eudicot Vitis vinifera (25), and the model crucifer A. thaliana, as well as B. rapa (9), B. oleracea (10, 11), and B. napus, are shown. Boulos Chalhoub et al. Science 2014;345: Published by AAAS

Fig. 2 The genome of the B. napus oilseed cultivar ‘Darmor-bzh’.The genome comprises 9 chromosomes belonging to the Cn subgenome and 10 to the An subgenome, scaled on the basis of their assembled lengths. Boulos Chalhoub et al. Science 2014;345: Published by AAAS

Fig. 3 HEs between B. napus chromosomes An2 and Cn2.(A) Coverage depth obtained along the An2 chromosome after mapping Illumina sequence reads from seven natural and one resynthesized B. napus genotypes (named on the right) to the reference genome of B. napus ‘Darmor-bzh.’ (B and C) Coverage depth obtained for Ar2 and Co2 chromosomes, respectively, after mapping >21 genome-equivalents of Illumina sequence reads from B. napus Darmor-bzh on the B. rapa and B. oleracea genome assemblies concatenated together. Boulos Chalhoub et al. Science 2014;345: Published by AAAS