Eli Asarian Riverbend Sciences Jacob Kann Aquatic Ecosystem Sciences, LLC. Ann St. Amand PhycoTech, Inc. Standardization and Long-term Trends in a Multi-Decadal Phytoplankton Database for Upper Klamath Lake, Oregon Funding: U.S. Bureau of Reclamation to Klamath Tribes Presented at: Society for Freshwater Science, Sacramento, CA 5/24/2016
Source: National Hydrography Dataset / USGS, Bureau of Transportation, National Atlas, Map by Jones and Trimiew Design. Klamath Basin
Upper Klamath Lake > Broad (~66,000 acres) and shallow (mean summer depth = 7 ft, max depth in Eagle Ridge = 58 ft) > Weak summer stratification, warm temperatures (peaks >25°C) > Hypereutrophic > Seasonal large blooms of nitrogen-fixing cyanobacteria Aphanizomenon flos-aquae > High pH, low dissolved oxygen, high ammonia Photos: Jacob Kann
Poor WQ linked to decline of federally listed shortnose (Chasmistes brevirostris) and Lost River (Deltistes luxatus) suckers Photos: USGS Shortnose sucker Lost River sucker Sucker larvae
Klamath Tribes Long-Term UKL Limnological Monitoring Program Includes: nutrients, light, YSI profile data, zooplankton and… phytoplankton 10 long-term sites Bi-weekly Mid-April to October (this effort)
Phytoplankton Sampling Methods Depth integrated over the entire water column Depth integrated over the entire water column Preserved with Lugol’s Iodine Preserved with Lugol’s Iodine Shipped to Laboratory Shipped to Laboratory
Laboratories Four phytoplankton labs: – Aqua I.D. – BSA Environmental –[ under-preserved] – Aquatic Analysts – PhycoTech
Ann St. Amand of PhycoTech Nationally recognized expert in plankton taxonomy JTG Chair, Plankton Section (10200), Standard Methods for the Examination of Waste Water and Water Participated in the European External Quality Assessment Trials Phytoplankton in Accordance with DIN38402 A 45; Certificate Received November By Barry H. Rosen and Ann St. Amand Recent coauthor of:
Need for Standardization Issues addressed: –Species identification –Levels of taxonomic detail –Outdated names –Counting units (e.g., cells vs. algal units) –Biomass methods No issue with Aphanizomenon ID
Tools for Standardization Review archived slides and consult taxonomic keys –confirm PhycoTech’s taxa identification Review previous taxonomic standardization efforts
Example of Inter-Lab QA Analysis of split samples sent to Aquatic Analysts and Phycotech Indicated good agreement for APFA colony density but cell size was flagged as a serious issue that would affect biovolume estimates (biovolume=size x density) AA used average cell size from previous measurements of northwestern US lakes as opposed to actual measurements.
Phytoplankton ID Issues Inconsistencies in how laboratories identified species; including dominant species as well as less common species. Example using the Diatom Stephanodiscus: Stephanodiscus medius and S. parvus
Detections Per Lab-Year Lab 1: Aqua ID Lab 2: BSA Lab 3: Aquatic Analysts Lab 4: PhycoTech Species Name Stephanodiscus astraea minutula Stephanodiscus minutulus Stephanodiscus minutula 1 Stephanodiscus parvus Stephanodiscus hantzschii Stephanodiscus medius Stephanodiscus niagarae
Biovolume Standardization Approach
PHYTO SEASONAL PATTERNS AND PRELIMINARY TRENDS
Seasonal pattern: major taxonomic groups
Trend: increased diatom dominance in spring Diatoms
Trend: decreased spring cyanobacteria Cyanobacteria
Seasonal pattern: N-fixers precede Microcystis
Species or Taxa analysis doesn’t include (high Microcystis years). Mann-Kendall Trend Tests Time Period
Conclusions Major inter-lab issues resolved Protocol for standardization of new data Preliminary trend analysis –Delayed onset of cyanobacterial bloom –Increased Microcystis Phase 2 analysis 2016/2017 –Update thru 2015 –Link plankton to environmental data (meteorology, nutrients, etc.)