AdvanSure ® for diagnosis of specific IgE Park, Jung-Won Allergy-Asthma Clinic, Severance Hospital

Food Allergy  Anaphylaxis type  Atopic dermatitis  Eosinophil associated gastrointestinal disorder Heterogeneity of Th2 cell: IL-5 + (IL-4 +, IL-5 + ) vs. IL-5 - (IL-4 +, IL-5 - )

Allergic eosinophilic gastroenteritis is associated with greater frequency of peanut specific IL-5 producing T cells Prussin C et al J Allergy Clin Immunol 2009

Mast Cell Bioactive mediators of three categories

Schematic of original Radioallergosorbent Test

 History  Resolution of skin symptoms with complete elimination  Development of symptoms following challenge  Skin test, sIgE test  DBPCFC Diagnosis of Food Allergy

Food Specific Ige (immuno-CAP RAST) values at /or above which there is a 95% risk of Clinical Allergy (no challenge necessary) Food Serum IgE (kIU/L) for 95% PPV Egg (child) >7 Cow’s milk (child) >15 Peanut >14 Fish >20

중요한 음식물 알레르겐 Classical allergens 땅콩 콩 달걀 우유 밀가루 : gliadine 견과류 : 호두, 아몬드 갑각류 : 새우, Lobster 생선 : 대구 메밀 Non-classical allergens (Oral allergy syndrome) 복숭아 사과 키위 생밤 참외

Allergen Panel of AdvanSure

Allergen Sources

Allergen Purification and Modification 기술

Allergen Quality Control

Production of Allergen-Coated Strips

 Chip reader 개발  Data processing program 개발

AdvanSure sIgE 진단 kit  Detection limit: 0.34 KU/L  Result range: Negative (<0.35 KU/L) ~ <100 KU/L  Serum requirement: 100 μL  Processing time: 3 hr

Analysis of discrepant results btw AdvanSure and RIDA compared with ImmunoCAP (n=633) Agreement rate btw AdvanSure and RIDA: 73.9% Oh EJ, et al Kor J Lab Med

AdvanSure® system showed good detection performance compared with ImmunoCAP® system in correlation and agreement. Upgrading of allergen item in panel may be needed. Conclusion