1. Inquiry 2/8/16 No warm-up today! Take out something to write with for our DNA Quiz. Put your lab book on the lab station by the fume hood. If you finish your quiz early, please log in to a netbook and start the yellow worksheet

2. DNA Quiz #2 Results Multiple ChoiceShort Answer A = 11-12 B = 9.5-10.5 C = 8.5-9 D = 7-8 Retakes for scores of 8 and under by the Wed. after break P. 3 Class Avg. = 8.1 (68%) P. 6 Class Avg. = 8.3 (69%) A = 12.5-14 B = 11-12 C = 10-10.5 D = 8.5-9.5 Retakes for scores of 9.5 and under by the Wed. after break P. 3 Class Avg. = 10.7 (76%) P. 6 Class Avg. = 9.6 (69%)

3. DNA Quiz Corrections 1. Re-Answer the short answer you missed after hearing my explanation 2. Copy this chart for YOUR test (A or B): Test A Test B TranscriptionTranslationMutations #1, 2, 5, 6#3, 4, 7, 8, 9, 12#10, 11 TranscriptionTranslationMutations #3, 4, 10, 12#1, 2, 5, 6, 7, 9#8, 11

4. DNA Quiz Corrections 3. Circle which ones you missed in your table. 4. If you missed half or more in a category, do the assignment on the half sheet for that category. 5. If you missed less than half in all categories, move on to the extra credit.

5. Biotechnology Topics What is biotechnology? Using technology to alter cells and DNA to improve the quality of human life (i.e. cure diseases, solve crimes, make organisms with new traits). 1. Stem Cells 2. Gel Electrophoresis 3. Designer Babies 4. Genetically Modified Organisms (GMOs)

7. Potential Uses of Stem Cells Embryonic Stem Cells could be used for: ◦ Growing replacement cells and organs Parkinson’s and Alzheimer’s diseases, spinal cord injuries, stroke, heart disease, type I diabetes, arthritis…

8. Watch this! Making new organs using stem cells or our own body cells? Why not? https://www.youtube.com/watch?v=TosV Ox7yjts https://www.youtube.com/watch?v=TosV Ox7yjts

9. Quick break Log in to a computer, please

10. What is Gel Electrophoresis? Gel Electrophoresis is the process of separating molecules based on size, charge, and shape ◦ Used to separate DNA, RNA and protein samples ◦ Gel box is filled with agarose gel, which is porous, and gel is covered with liquid buffer ◦ Samples are loaded into wells in the gel and voltage is turned on

12. What is Gel Electrophoresis? Samples are pulled toward + or – end of box depending on charge Large and bulky molecules don’t travel as far as small and smooth molecules Neg. End (-) - +

13. Let’s Learn More! Complete the Gel Electrophoresis online assignment ◦ Learn.genetics.utah.edu ◦ Go to Virtual Labs (bottom of page) ◦ Select “gel electrophoresis” It’s homework if you don’t finish.

14. Pipette Practice Micropipettes are used to measure TINY amounts of liquid (1 microliter = 1/1000 th of a mL!) Make a “gel” out of playdough Put the comb in it to make indentations Practice pipetting the practice liquid into the holes