1. BBa_J07008 BBa_J07009

2. BBa_J07010 CELL

3. Y Y Transmembrane protein Sensor antibodies scFv

4. ANTIGEN Y Y Y Y Y Y Y Primary antibodies

5. Y Y ANTIGEN Y Y

6. Y Y Y Y

7. Head Receiver Unit Receiver 1 Receiver 2 Signal Processor Actuator Input Output dimerization conformational change Head Receiver Unit Input Device

8. fecA Signal Processor Input dimerization conformational change Input Device scFv BBa_J07012 or BBa_J07013 or BBa_J07014 toxR BBa_J07009 scFv BBa_J07012 or BBa_J07013 or BBa_J07014 GFP BBa_E0040

9. 1.Utilizes the honing mechanism of antibodies for target detection. 2.Standardized technology for detecting primary antibodies. 3.Programmable biological sensor with interchangeable parts. 4.Generalized system able to perform biospecific, and highly sensitive target recognition.

10. Think of it this way… Biological Power Drill ?

11. Standard chuck for attachments Interchangeable bits with standard connectors to chuck.

12. Y Y Y Y Y Standard sensor for primary antibody attachment Interchangeable primary antibodies with recognizable constant region to variable region of secondary antibody sensor. “power drill” “drill bits”