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Amino Acids and Proteins Amino Acids. L-isoleucine.

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Presentation on theme: "Amino Acids and Proteins Amino Acids. L-isoleucine."— Presentation transcript:

1 Amino Acids and Proteins Amino Acids

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3 L-isoleucine

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5 zwitterion

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9 Synthesis of Amino Acids Strecker Synthesis

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11 Gabriel Synthesis

12 Biosynthesis

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14 Transamination

15 Polypeptides and Proteins

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20 Carboxypeptidase

21 Trypsin, chymotrypsin

22 BrCN

23 Complete hydrolysis of a polypeptide gives: Gly, 3 Leu, 2 Tyr, 2 Phe, 2 Lys, Ser, 2 Met, Ala, Val, Ile, Arg, Pro Cyanogen bromide generates a tripeptide, a heptapeptide, and an octapeptide; each is sequenced: Leu-Pro-Phe Gly-Leu-Trr-Phe-Lys-Ser-Met Ala-Leu-Tyr-Lys-Val-Ile-Arg-Met

24 Partial hydrolysis with trypsin gives a tripeptide, a tetrapeptide, a pentapeptide, and a hexapeptide; each is sequenced: Val-Ile-Arg Met-Leu-Pro-Phe Gly-Leu-Tyr-Phe-Lys Ser-Met-Ala-Leu-Tyr-Lys What is the sequence for the original polypeptide?

25 Polypeptide and Protein Synthesis

26 Protecting Groups

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32 Activating Groups

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37 Automated Synthesis

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41 Secondary Structures of Polypeptides and Proteins

42 Pleated Sheet

43  -Helix

44 Tertiary Structures of Polypeptides and Proteins

45 Enzymes Carboxypeptidase A

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