1. Department of Medical Imaging
Noninvasive Measuring Brain pH and Biomarker of Brain Tissue Using MR Technology
Ren-Hua Wu, Dr. med.
Department of Medical Imaging
Shantou University
China

2. Background
Our ongoing studies

3. Background: Normal or Abnormal?
T1WI
T2WI
MRS

4. T1WI
T2WI
DWI
5h after ictus
ADC
MRS

5. Glioma or acoustic neuroma?
Pilocytic
astrocytoma

6. Differential diagnosis
Biopsy
CT, MR, PET/CT
Contrast media

7. 1H谱
1H MRS

8. 31P MRS

9. MRS
Multi-voxel
Single voxel

10. Background Data from Louis N. Manganas, et al. Science 318, 980 (2007)
Identify Neural Progenitor Cells
Data from Louis N. Manganas, et al.
Science 318, 980 (2007)

11. Detecting cell apoptosis by MRS
Control
Cell apoptosis
Cao Z, et al. World J Gastroenterol. 2008;14(24):3891-6

12. Non-invasive detection of hippocampal sclerosis
Data from Hammen T, et al. NMR Biomed Jul;21(6):

13. Background：Benign or Malignant？
ET/CT

14. Background-pH imaging
Gallagher FA, et al.
Magnetic resonance imaging of
pH in vivo using hyperpolarized
13C-labelled bicarbonate.
Nature. 2008;453(7197):940-3.

15. Background: 1H pH imaging
Data from Zhou JY, et al.
Nature Medicine. 2003; 9(8):

16. Background: 31P pH imaging
Wu RH, et al. Chwee Teck Lim, James C.H. Goh (Eds.): ICBME 2008, Proceedings 23, pp , 2009

17. Our ongoing studies
1.28 ppm biomarker is dominant in mesenchymal stem cells and adipogenic differentiation induced.
1.28 ppm signals is not specific for Neural Progenitor Cells

18. Mesenchymal stem cell before and after differentiation

19. B-1 A
B-2 B
Fig.B.The spectra of control group (A) and model group (B).
The spectra of glutamate at 2.35 ppm is lower than control group.
model for Alzheimer's disease

20. control
60 days
70 days

21. A：Mouse brain B：Human glioma
C：human esophagus carcinoma D： Mesenchymal stem cell

22. Our ongoing studies：pH imaging in model
FSE sequence

23. Our ongoing studies：pH imaging in model

24. pH Imaging
T2WI
pH-sensitive imaging
pH-sensitive imaging with color

25. pH sensitive imaging: GRE-MT -200
Before editing
After editing

26. Acknowledgements National Natural Science Foundation of China (NSFC)
Li Ka Shing Foundation

27. Acknowledgements