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Human platelets synthesize and express functional tissue factor by Olga Panes, Valeria Matus, Claudia G. Sáez, Teresa Quiroga, Jaime Pereira, and Diego.

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Presentation on theme: "Human platelets synthesize and express functional tissue factor by Olga Panes, Valeria Matus, Claudia G. Sáez, Teresa Quiroga, Jaime Pereira, and Diego."— Presentation transcript:

1 Human platelets synthesize and express functional tissue factor by Olga Panes, Valeria Matus, Claudia G. Sáez, Teresa Quiroga, Jaime Pereira, and Diego Mezzano Blood Volume 109(12):5242-5250 June 15, 2007 ©2007 by American Society of Hematology

2 TF mRNA in human platelets and PBMCs. Platelet and PBMC RNAs were extracted before and after stimulation with 5 μM TRAP for 15 minutes. Olga Panes et al. Blood 2007;109:5242-5250 ©2007 by American Society of Hematology

3 Immunoprecipitation and biotinylation of TF in platelet membranes. Olga Panes et al. Blood 2007;109:5242-5250 ©2007 by American Society of Hematology

4 Confocal immunodetection of TF and GPIbα in human, nonpermeabilized platelets. Olga Panes et al. Blood 2007;109:5242-5250 ©2007 by American Society of Hematology

5 De novo synthesis of TF by platelets and PBMCs. Platelets or PBMCs with or without pretreatment with puromycin, actinomycin D, DRB, or tunicamycin were incubated with [35S]- methionine, and activated with TRAP or LPS. The platelet membranes before and after... Olga Panes et al. Blood 2007;109:5242-5250 ©2007 by American Society of Hematology

6 Confocal immunofluorescence of TF and GPIbα in nonstimulated PBMC preparations. Olga Panes et al. Blood 2007;109:5242-5250 ©2007 by American Society of Hematology

7 Confocal immunofluorescence of TF protein expressed by LPS-stimulated PBMC preparations. Olga Panes et al. Blood 2007;109:5242-5250 ©2007 by American Society of Hematology


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