1. DNA Replication Copying the genetic code

2. DNA Replication  Unpack the Chromosomes  Unzip the DNA  Base pairing at the replication fork  Leading strand vs Lagging strand

3. Making Copies

5. copycopied during cell division Sister Chromatids (identical copies)

7. Accessing the DNA inactive DNA (deactivated or mitotic) DNA in use

8. Visualization of DNA Packing  For this video, visit: http://www.hhmi.org/biointeractive/dna /DNAi_packaging_vo2.html http://www.hhmi.org/biointeractive/dna /DNAi_packaging_vo2.html http://www.hhmi.org/biointeractive/dna /DNAi_packaging_vo2.html

9. Accessing the DNA

11. DNA Structure

12. Complementary Base Pairing

13. Identical Parent and Daughter Strands

14. Replication Bubbles helicase 5’ 3’ 5’

15. Replication Bubbles 5’ 3’ 5’

16. Replication Bubbles 5’ 3’ 5’

17. Replication Bubbles copies Replication Fork 5’ 3’ 5’

18. Replication Bubbles

19. Releasing the Tension

20.  Topoisomerase cleaves and uncoils DNA ahead of helicase  Single-stranded DNA binding proteins (SSB) prevent DNA from coiling back up

21. DNA Replication Primase RNA primer DNA Polymerase III 5’ 3’ 5’

22. DNA Replication  The leading strands grow toward the replication fork Leading Strands 5’ 3’ 5’

23. DNA Replication 5’ 3’ 5’

24. DNA Replication Lagging Strands  The lagging strands grow away from the replication fork 5’ 3’ 5’

25. DNA Replication 5’ 3’ 5’ DNA Polymerase I DNA Ligase

26. DNA Repliaction 5’ 3’ 5’

27. DNA Repliaction Okazaki Fragments 5’ 3’ 5’

31. The Whole Process in 3D  For this video, visit:  http://www.hhmi.org/biointeractive/dna /DNAi_replication_schematic.html http://www.hhmi.org/biointeractive/dna /DNAi_replication_schematic.html http://www.hhmi.org/biointeractive/dna /DNAi_replication_schematic.html

32. The Whole Process in 3D  For this video, visit: http://www.hhmi.org/biointeractive/dna/ DNAi_replication_vo2.html http://www.hhmi.org/biointeractive/dna/ DNAi_replication_vo2.html