2. Fig. 5.3

3. Transforms R cells

4. Fig. 5.5

6. Uracil (U) Five Fig. 5.7

9. (a.k.a. polarity) Sequence: 5’ ATGCA 3’

11. Fig. 5.6

12. 34 Fig. 5.9

14. 34 Fig. 5.9

16. Fig. 5.14

17. Models for DNA Replication Daughter Helix: semi-conservative: one old/one new strand conservative: two new strands dispersive: mix of old & new

18. Fig. 5.16 or

19. Fig. 5.17

20. Bacterial Chromosome Eukaryotic Chromosome

21. Initiation: Opening and Stabilizing the Replication Bubble origin of replication Initiator Protein DNA Helicase Single Stranded Binding Proteins

22. DNA Polymerase III 10 protein subunits Restrictions on Nucleotide Addition –Copies only single stranded DNA –Can add only to pre-existing chains –Adds only in the 5’ to 3’ direction

23. Fig. 5.17

24. AGGCCTCGAATTCGTATAGCTTTCAGA5’3’ UUAAGCAUAU5’3’ WHAT WOULD BE THE RNA PRIMER’S SEQUENCE? Alternatively: 5’ UAUACGAAUU 3’

25. Elongation Phase of DNA Replication Nucleotide Selection –via complementary base pairing Phosphodiester Bond Forms Helicase Continues Moving –opening the Replication bubble DNA Polymerase Trails Behind –adding more nucleotides

26. DNA Polymerase III 10 protein subunits Restrictions on Nucleotide Addition –Copies only single stranded DNA –Can add only to pre-existing chains –Adds only in the 5’ to 3’ direction

27. 3’ end5’ end

29. Origin 5’ 3’ 5’ 3’ leading strand lagging strand movement of growing fork

30. 1. Bidirectional replication 2. Leading Strand Continuous 3. Lagging Strand Discontinuous Okazaki Fragments 5’3’ 5’3’