1. Testing for Organic Macromolecules

2. What are Macromolecules? Large molecules made of smaller molecules Or Small molecules Or Macromolecule

3. What do we mean by Organic? Compounds that contain the atom Carbon are considered organic. All living things are made of organic molecules!

4. There are four classes of Organic Compounds 1.Carbohydrates 2.Proteins 3.Lipids 4.Nucleic acids

5. Indicators Are substances used to test for the presence of specific chemical compounds. Have a known response to specific chemical compounds. Indicate whether a chemical compound is present in the solution you are testing.

6. The slides that follow have instructions for using specific indicators to test for four specific organic compounds! Use these notes as procedures for your lab today and for your final lab write up. **Note: You will find out which compound each indicator tests for next week when we take notes on each category of organic compounds.

7. Indicator: BROWN PAPER 1.Dip the clean end of a cotton swab into the solution you are testing, swirl it around. 2. Wipe a thin layer of the SOLUTION you are testing ONTO BROWN PAPER. If you put to much on, dab it off with paper towel 3. WAIT FOR IT TO COMPLETELY DRY (do this test first so you have plenty of drying time) 4. A TRANSLUCENT SPOT (LIGHT SHINES THROUGH) is a positive test result. ***Any other change or no change is a negative result!

8. 1.Wash your test tube! 2.Stir the solution you are testing, then add 3 ml to your test tube. 3.Add 15 drops of Benedicts (the indicator) to 3 ml of the solution in the test tube. 4.Swirl test tube to mix. 5. Heat solution, in a water bath, for approximately 1-2 min. 6.If the solution turns orange, this is a positive result. ***Any other change or no change is a negative result! Indicator: BENEDICT’S

9. Indicator: BIURET’S 1.Wash your test tube! 2.Stir the solution you are testing, then add 3 ml to your test tube. 3.Add 10 drops of Biuret’s to 3 ml of solution in the test tube. 4.Swirl test tube to mix. 5.A positive test result is if the solution turns purple. (hold up to white paper if not sure) ***Any other change or no change is a negative result!

10. Indicator: IODINE 1.Wash your test tube! 2.Stir the solution you are testing, then add 3 ml to your test tube. 3.Add 10 drops of Iodine to 3 ml of solution in the test tube. 4.Swirl test tube to mix. 5.A positive test result is if the solution turns dark blue or black. ***Any other change or no change is a negative result!

11. Use the graduated cylinder to measure out 3 ml. After measuring, put the solution you are testing into the test tube, then add indicator and swirl! A A