1. Prokaryotes: Kingdoms Archaebacteria and Eubacteria Chapter 18

2. Daily Objective Investigate Prokaryotes and their two Domains: Domain Archaea and Domain Bacteria

3. Bacteria are the most numerous organisms on Earth Earliest life forms Contain no nucleus or membrane bound organelles Most are unicellular FAST FACTS

4. Prokaryotes are divided into 2 domains. Domain Archaea and Domain Bacteria Review Domain Archaea live in extreme enviornments. They have been found to have similarites with eukaryotic cells. i.e. the ribosomal proteins Domain Bacteria live in nearly every environment on Earth. Their cell walls contain peptidoglycan.

5. Daily Objective Review Characteristics of Prokaryotes

6. Domain Archaebacteria “ancient bacteria” Cell walls without peptidoglycan Subdivided into 3 groups based on their habitat– methanogens, thermoacidophiles, & extreme halophiles

7. Domain Bacteria “true bacteria” Most bacteria in this kingdom Come in 3 basic shapes cocci (spheres), bacilli (rod-shaped), spirilla (corkscrew shape) Most are heterotrophic Can be aerobic, or anerobic

8. Gram Staining Developed in 1884 by microbiologist Hans Gram Bacteria are stained purple with dye and iodine, rinsed with alcohol to decolorize, then restained with dye. Bacterial cell walls either stain purple or reddish-pink

9. Gram +/- Gram (+) Bacteria Stain purple Thick layer of peptidoglycan (protein sugar complex in cell walls) Examples: streptoccous, staphylocci Can be treated with penicillin (antibiotics) Gram (-) Bacteria Stain reddish-pink Thin layer of peptidoglycan Antibiotic resistant Examples; Rhizobacteria, Rickettsia (Lyme disease)

10. Prokaryote Structure

11. Prokaryote Characteristics Bacteria can be identified by their shape, cell wall, and movement. Shape

12. Prokaryote Characteristics- cell walls All bacterial cells have peptidoglycan (sugar and protein) in their cell walls -can identify bacteria by gram staining. Bacteria with a large amount of peptidoglycan appear dark purple (gram +) Bacteria with a lipid layer has less peptidoglycan, and will stain reddish pink (gram-)

13. Reproduction of Prokaryotes Most bacteria reproduce asexually by binary fission- chromosome replicates then the cell divides Bacteria can sexually reproduce by conjugation- two bacteria from a conjugation bridge or tube between them.

14. Which method of Reproduction?

15. Metabolism of Prokaryotes Prokaryotes can obtain their energy in multiple ways HeterotrophsPhotoAutrophsChemoautotrophs Do not synthesize own food. Obtain energy by decomposing organic molecules. Photosynthetic. Release oxygen into the atmosphere Do not require light for energy. They break down and release inorganic compounds.