1. 2015 GEM Midwest Trial Results & New Releases C. Gardner, F. Engstrom, M. Peters, M. Shen USDA-ARS GEM December 9, 2015 G ermplasm E nhancement of M aize

2. GEM Released Lines 286 Releases from the Ames and Raleigh programs are available to GEM Cooperators; 301 total released with public cooperators Twelve GEM lines are proposed for release from the Ames program for 2016 planting G ermplasm E nhancement of M aize

3. Pathology Trials 2015 NLB, SLB (USDA-ARS-PSRU Raleigh, NC) Aflatoxin (USDA-ARS CHPRRU, MS State) NLB and Diplodia (DuPont/Pioneer Hi-Bred) NLB, GLS, and Goss’s Wilt (Professional Seed Research) Army Worm/CEW (3 rd Millennium Genetics G ermplasm E nhancement of M aize

4. Allelic Diversity(AD) and Double Haploids (DH) Update 2015 204 BGEM DH lines jointly released in 2014 with Iowa State University’s Doubled Haploid Facility, representing ~52 races 22 sets of these lines have been distributed to date, plus numerous selections 140 DH 1 lines were increased to DH 2 240 DH 0 lines were increased to DH 1 G ermplasm E nhancement of M aize

5. 7,610 nursery, and 3,697 isolation block rows at Ames Late planting and too much rain during pollinating complicated coverage of pollinations Additional nursery and isolation support from 13 other US companies in winter nurseries Nursery and Isolations Blocks G ermplasm E nhancement of M aize

6. Commercial Checks 2015 HybridMidwest RM (in our 2015 tests) MBS3644/MBS5411᷉109 MBS9508/MBS2655᷉107 34R65᷉109 Pioneer 33F85᷉109 Pioneer 31D58᷉116 G ermplasm E nhancement of M aize

7. GEM CodePedigreeRaceCountryType GEMS-0275((KO679Y/GEMS- 0115)/GEMS-0162)-008- 001 ((25% KO679)(25% DKB844)) / (25% Cuba117) South Africa, Brazil, Cuba 25% tropical GEMS-0276(53SS4/GEMS-0026)-SIB- B-003 (25% exotic donated by coop) / (50% DK370A) Brazil37.5% tropical GEMS-0277(53SS4/GEMS-0031)-SIB- B-039 (25% exotic donated by coop) /(50% DK370A) Brazil37.5% tropical GEMS-0278(GEMS-0115/S49w)-B-B- 036 25% DKB844Brazil12.5% tropical GEMS-0279(GEMS-0176/S49w)-B-B- 028 25% Cuba164, CriolloCuba25% tropical GEMS-0280BR52051:S172641-B-018PI 583917, Dente AmareloBrazil25% tropical 2016 GEM Releases - Ames G ermplasm E nhancement of M aize

8. GEMS-0281BR52051:S172641-B-054PI 583917, Dente AmareloBrazil25% tropical GEMS-0282CHIS740:S(PHW17)(87916 W)-126-001 PI583890,Tuxpeno/OlotilloChile50% temperate GEMS-0283UR11002:S1409-068-001PI 383923, Dente Branco Landrace Uruguay50% temperate GEMS-0284AR17056:S1217-B-02550% PI 493039, Cristalino Colorado Argentina50% temperate GEMN-0285(GEMN- 0117/DKXL212:N11a-139- 001-001-B-B-B-B-B-B)-B-B- 019 (25% PI 484028, Tuson/Canilla Landrace from Dominican Republic)/(25% DKXL212 Dominican Republic 25% tropical GEMN-0286MDI022:N(PHG35)(GEMN- 0097)-072-001 (PI 571994, Cuban yellow population) / (25% PI 536622, FS8B(T)) Peru18.3% exotic G ermplasm E nhancement of M aize

9. GEMS-0275: ((KO679Y/GEMS-0115)/GEMS-0162)-008-001 PedigreePedigree BackgroundNursery ObservationsGDU Pol. ((KO679Y/GEMS-0115)/GEMS-0162)-008- 001/LH287 ((25% KO679)(25% DKB844))/(25%Cuba117); 25% exotic NLF-free in 2015 nursery; good ears, grain quality, stalks; semi-flint K 1438 TestPedigree % CheckYieldVarianceMoist Yield/ MoistTWT Plant Height Ear Height Final Stand Stalk Lodging Root Lodging 141407((KO679Y/GEMS-0115)/GEMS-0162)-008-001/LH287101.5182.954822.48.254.6991.412.0 1564204((KO679Y/GEMS-0115)/GEMS-0162)-008-001/LH28798.5195.581219.610.053.8270120985.210.3

10. GEMS-0276: (53SS4/GEMS-0026)-SIB-B-003 TestPedigree % CheckYieldVarianceMoist Yield/ MoistTWT Plant Height Ear Height Final Stand Stalk Lodging Root Lodging 141401(53SS4/GEMS-0026)-SIB-B-003/LH28793.2186.031021.48.853.0981.38.3 1564202(53SS4/GEMS-0026)-SIB-B-003/LH28799.7198.243821.19.654.4290150913.525.4 PedigreePedigree BackgroundNursery ObservationsGDU Pol. (53SS4/GEMS-0026)-SIB-B-003 (25% exotic donated by coop)/(50% DK370A); 37.5% exotic good late NLF; small ear, good grain quality, stalks; semi-dent K 1349

11. GEMS-0277: (53SS4/GEMS-0031)-SIB-B-039 TestPedigree % CheckYieldVarianceMoist Yield/ MoistTWT Plant Height Ear Height Final Stand Stalk Lodging Root Lodging 141402(53SS4/GEMS-0031)-SIB-B-039/LH28793.4175.542819.19.253.81011.119.5 1564202(53SS4/GEMS-0031)-SIB-B-039/LH28797.6194.280020.99.554.2280100931.83.2 PedigreePedigree BackgroundNursery ObservationsGDU Pol. (53SS4/GEMS-0031)-SIB-B-039 (25% exotic donated by coop)/(50% DK380, Brazil); 37.5% exotic no rust; good late NLF; late, green; good ears, grain quality, stalks; dent/semi-dent K 1381

12. GEMS-0278: (GEMS-0115/S49w)-B-B-036 TestPedigree % CheckYieldVarianceMoist Yield/ MoistTWT Plant Height Ear Height Final Stand Stalk Lodging Root Lodging 141404(GEMS-0115/S49w)-B-B-036/LH28789.7183.228820.88.853.31020.02.2 1564203(GEMS-0115/S49w)-B-B-036/LH287100.5190.485419.99.755.4285135984.03.6 PedigreePedigree BackgroundNursery ObservationsGDU Pol. (GEMS-0115/S49w)-B-B-036(25% DKB844)/temperate; 12.5% exotic NLF below avg in nursery; short, good stay green, ears; ok husk cover; semi-flint K; 1481

13. GEMS-0279: (GEMS-0176/S49w)-B-B-028 TestPedigree % CheckYieldVarianceMoist Yield/ MoistTWT Plant Height Ear Height Final Stand Stalk Lodging Root Lodging 141403(GEMS-0176/S49w)-B-B-028/LH28792.0179.28821.28.654.0911.82.8 1564203(GEMS-0176/S49w)-B-B-028/LH287101.9192.983319.310.256.5275135990.80.0 PedigreePedigree BackgroundNursery ObservationsGDU Pol. (GEMS-0176/S49w)-B-B-02825% Cuba164/temperate; 25% exotic NLF below avg in nursery; good ears, stalks; semi- flint K 1437

14. GEMS-0280: BR52051:S172641-B-018 TestPedigree % CheckYieldVarianceMoist Yield/ MoistTWT Plant Height Ear Height Final Stand Stalk Lodging Root Lodging 141406BR52051:S172641-B-018/LH28796.9182.160223.28.052.71000.627.5 1514203BR52051:S172641-B-018/LH287103.3204.913221.89.655.1280135971.23.2 PedigreePedigree BackgroundNursery ObservationsGDU Pol. BR52051:S172641-B-018 (PI 583917, Brazilian Dente Amarelo) / temperate; 25% exotic excellent late health; dense, leafy habit; early flowering; large ears, average grain qul; dent K; 1431

15. GEMS-0281: BR52051:S172641-B-054 TestPedigree % CheckYieldVarianceMoist Yield/ MoistTWT Plant Height Ear Height Final Stand Stalk Lodging Root Lodging 141406BR52051:S172641-B-054/LH28792.2173.494221.78.252.71000.311.8 1514203BR52051:S172641-B-054/LH28799.9198.1119620.99.755.42901251001.20.3 PedigreePedigree BackgroundNursery ObservationsGDU Pol. BR52051:S172641-B-054 (PI 583917, Brazilian Dente Amarelo) / temperate; 25% exotic good staygreen, but lot of common rust; good ears, grain quality; ok husk cover; dent K; 1411

16. GEMS-0282: CHIS740:S(PHW17)(87916W)-126-001 TestPedigree % CheckYieldVarianceMoist Yield/ MoistTWT Plant Height Ear Height Final Stand Stalk Lodging Root Lodging 141312CHIS740:S(PHW17)(87916W)-126-001/WT99387.9163.715225.46.552.1903.119.0 1564201CHIS740:S(PHW17)(87916W)-126-001/WT99399.8191.6131823.98.055.12902759719.410.8 PedigreePedigree BackgroundNursery ObservationsGDU Pol. CHIS740:S(PHW17)(87916W)-126-001 (PI583890, Chilean Tuxpeno/Olotillo Landrace)/temperate; 100% temperate tall, late, green; below avg stks; seg W/Y grain; W cob; semi- flint; there are two sister lines that have pure white K, more variable yld record; 1450

17. GEMS-0283: UR11002:S1409-068-001 TestPedigree % CheckYieldVarianceMoist Yield/ MoistTWT Plant Height Ear Height Final Stand Stalk Lodging Root Lodging 145301UR11002:S1409-068-001/LH28792.5188.610620.49.355.1962.911.9 1564201UR11002:S1409-068-001/LH28798.0188.333020.59.257.1290120962.21.5 PedigreePedigree BackgroundNursery ObservationsGDU Pol. UR11002:S1409-068-001 (PI 383923, Dente Branco Landrace, Uruguay)/temperate; 100% temperate health average; short, low ear ht; moderate ear size, good grain quality; semi-flint K; 1369

18. GEMS-0284: AR17056:S1217-B-025 TestPedigree % CheckYieldVarianceMoist Yield/ MoistTWT Plant Height Ear Height Final Stand Stalk Lodging Root Lodging 145404AR17056:S1217-B-025/MBS8814106.8209.657624.88.452.0250140971.920.2 1564204AR17056:S1217-B-025/MBS8814105.1208.657722.49.551.9280155963.32.9 PedigreePedigree BackgroundNursery ObservationsGDU Pol. AR17056:S1217-B-025 50% PI 493039, Cristalino Colorado; 100% temperate line tall, hi-eared; ECB, tops out; big ears, good grain quality; average stalks; dent K; 1380

19. GEMN-0285: (GEMN-0117/DKXL212:N11a-139-001-001-B-B-B-B-B-B)-B-B-019 TestPedigree % CheckYieldVarianceMoist Yield/ MoistTWT Plant Height Ear Height Final Stand Stalk Lodging Root Lodging 145403 (GEMN-0117/DKXL212:N11a-139-001-001-B-B-B-B-B-B)-B-B-019/MBS3520 93.7169.698519.48.853.91901051041.811.0 1564204 (GEMN-0117/DKXL212:N11a-139-001-001-B-B-B-B-B-B)-B-B-019/MBS3520 97.0192.121417.711.054.13001501001.72.5 PedigreePedigree BackgroundNursery ObservationsGDU Pol. (GEMN-0117/DKXL212:N11a-139-001- 001-B-B-B-B-B-B)-B-B-019 (25% PI 484028, Tuson/Canilla Landrace from Dominican Republic)/(25% DKXL212); 25% exotic NLF-free in 2015 nursery; hypersensitive rust rxn; slender ear, 12 Kernel rows; early flowering; good ears, grain quality, stalks; soft dent K; 1407

20. GEMN-0286: MDI022:N(PHG35)(GEMN-0097)-072-001 TestPedigree % CheckYieldVarianceMoist Yield/ MoistTWT Plant Height Ear Height Final Stand Stalk Lodging Root Lodging 141306MDI022:N(PHG35)(GEMN-0097)-072-001/HC3393.9156.493419.98.054.91013.11.7 141310MDI022:N(PHG35)(GEMN-0097)-072-001/HC3398.0207.811020.410.357.21081461002.53.1 1564201MDI022:N(PHG35)(GEMN-0097)-072-001/HC33105.4202.546120.49.957.2330155943.35.8 PedigreePedigree BackgroundNursery ObservationsGDU Pol. MDI022:N(PHG35)(GEMN-0097)-072-001 (PI 571994, Cuban yellow population)/(PHG35)(25% PI 536622, FS8B(T)); 18.37% exotic excellent late health, NLF-free in nursery; low ear ht; long ear, good grain quality, large dent K; 1526

21. GEM Field Days Raleigh, NC – TSG Meeting and Field Day, August 3-6 Ames, IA, September 22 Beijing, China G ermplasm E nhancement of M aize

22. Public Cooperator Reports Iowa State University- Germplasm Improvement for Starch and Bio-fuel Truman State U.- Specialty Starch Germplasm to Optimize Grain Quality & Yield G ermplasm E nhancement of M aize

23. Thank you for your past and future contributions ! The good news: a new maize geneticist will coordinate the Ames GEM program early in 2016 We look forward to working together with our new coordinator and colleagues at Raleigh to enhance collaborative activities with all public and private sector cooperators.