1. Rochester Data in Sesame Logging in to Genie to handle 96-well plates http://kamba.nmrfam.wisc.edu/Sesame

2. Rochester Data in Sesame Search for plate by owner: select from menu

3. Rochester Data in Sesame Search by owner for plates: enter, or leave, name

4. Rochester Data in Sesame Search by owner for plates: expand tree

5. Rochester Data in Sesame Select workgroup = one step for one plate

6. Rochester Data in Sesame General info for workgroup: ID, protocol, date...

7. Rochester Data in Sesame ORFs (Targets): properties and actions

8. Rochester Data in Sesame Enlarged view of column headers

9. Rochester Data in Sesame Select wells by position or name to record results

10. Rochester Data in Sesame Invert selection with 1 click

11. Rochester Data in Sesame Add action = result or check point

12. Rochester Data in Sesame Lamp: Large scale cell growth & protein prep Protocol Tree Seleno Methionine (yes or no) Batch ID (starting from 1) Cell Growth : Growth Rate Methods: Temperature, Volume, Medium, Aeration Induction: OD at induction, Final OD, Wet Weight, Expression level (low, whopping, etc) Methods: IPTG, Autoinduction Solubilization : Fraction solubilized Methods: Salt, pH, Detergent, Other Purification : Purity from Columns 1,2,3; Yield as % of original and final mass Methods: For Columns 1,2,3 - Type, Load, Wash, Reaction, Elution Cleavage (yes or no): Percent cleaved Methods: Column or solution stage, Enzyme, solution, Time, Temperature Final Desalting Shipping: Concentration, mg/ml, Buffer To Buffalo: Volume, Temperature To Seattle: Total volume, Volume per tube, Temperature