1. Optimization Lab Kalim Kassim, Diana Dominguez, Nafisa Ibrahim

2. Purpose: To obtain the optimal concentration of substrate hydrogen peroxide To obtain the optimal concentration of substrate hydrogen peroxide

3. Materials Pipettes (2) Pipettes (2) Beakers (2) Beakers (2) Test tubes (6) Test tubes (6) Peroxidase Peroxidase Test tube rack Test tube rack H2O2 1% concentration (90 ml) H2O2 1% concentration (90 ml) H2O (30 ml) H2O (30 ml) Tweezers Tweezers Stop watch Stop watch Paper disks (6) Paper disks (6)

4. Procedure 1. Obtain 6 test tubes and a test tube rack 1. Obtain 6 test tubes and a test tube rack 2. Label the 6 test tubes according to the 6 samples 2. Label the 6 test tubes according to the 6 samples 3. Obtain 2 pipettes 3. Obtain 2 pipettes 4. Label one pipette for water and the other for H2O2 4. Label one pipette for water and the other for H2O2 5. In the first test tube, put in 20 ml of H2O2 and 0 ml water 5. In the first test tube, put in 20 ml of H2O2 and 0 ml water 6.Obtain a paper disk 6.Obtain a paper disk 7. Dip it into the enzyme peroxidase 7. Dip it into the enzyme peroxidase 8. Place the wet paper disk into the test tube 8. Place the wet paper disk into the test tube 9. Time how long it takes to descend and how long it takes to ascend 9. Time how long it takes to descend and how long it takes to ascend 10. The test is over when the paper disk reaches the top 10. The test is over when the paper disk reaches the top 11. Repeat steps 1 -10 for the other different solutions using different quantities of H2O2 and water ( decrease the quantity of H2O2 by 2 ml every time and increase the quantity of water by 2 ml every time, until they reach even) 11. Repeat steps 1 -10 for the other different solutions using different quantities of H2O2 and water ( decrease the quantity of H2O2 by 2 ml every time and increase the quantity of water by 2 ml every time, until they reach even)

5. Observations: Ascension + Dissension

7. Ascension vs. Dissension

9. Analysis After completing this lab, it was found that the optimal concentration of H202 is when there is a 9:1 ratio of H202 to water. In this lab, it was the solution with18 ml of H202 and 2 ml of water. This test also took the longest time to descend and ascend, at 24 seconds. After completing this lab, it was found that the optimal concentration of H202 is when there is a 9:1 ratio of H202 to water. In this lab, it was the solution with18 ml of H202 and 2 ml of water. This test also took the longest time to descend and ascend, at 24 seconds. According to the rest of the observations, the solution with 14 ml of H2O2 and 6 ml of water was the fastest to descend and ascend, at 15 seconds, as it stopped midway through the test tube and began to ascend. According to the rest of the observations, the solution with 14 ml of H2O2 and 6 ml of water was the fastest to descend and ascend, at 15 seconds, as it stopped midway through the test tube and began to ascend. For the last 2 solutions, (12 ml of H202 and 8 ml of water) and (10 ml of each), both times were the same at 20 seconds. By looking at this, all other tests after this will be around 20 seconds, thus making solution number 2 the optimal one. For the last 2 solutions, (12 ml of H202 and 8 ml of water) and (10 ml of each), both times were the same at 20 seconds. By looking at this, all other tests after this will be around 20 seconds, thus making solution number 2 the optimal one. Sources of error in this lab that may have altered the data may have been: failure to record the time when the paper disc was idle at the bottom of the test tube. During the experiment, some paper discs remained idle at the bottom of the test tubes for a period of time. This time was not recorded because the test was presumed a fail. After a period of time the paper disc rose. These tests were done again but the time at the bottom of the test tube was still not recorded. If this time was recorded all data would have changed and been more accurate. Sources of error in this lab that may have altered the data may have been: failure to record the time when the paper disc was idle at the bottom of the test tube. During the experiment, some paper discs remained idle at the bottom of the test tubes for a period of time. This time was not recorded because the test was presumed a fail. After a period of time the paper disc rose. These tests were done again but the time at the bottom of the test tube was still not recorded. If this time was recorded all data would have changed and been more accurate. Another source of error present in this lab was that there were no tests that used the H2O2 6% concentration. If tests were done with this concentration of H2O2, more data would have been present and a comparison could have been done. Another source of error present in this lab was that there were no tests that used the H2O2 6% concentration. If tests were done with this concentration of H2O2, more data would have been present and a comparison could have been done. After concluding all this, this makes the lab data totally theoretical. After concluding all this, this makes the lab data totally theoretical.