1. Cultured in vitro human autologous epidermis for clinical applications Justyna Drukała Laboratory of Cell and Tissue Engineering Department of Cell Biology Faculty of Biochemistry, Biophysics & Biotechnology Jagiellonian University

2. Skin-replecement product technology
0.5 cm2 patients own skin biopsy
epidermal cells culturing in vitro
prepared cells transplanted back on to the same patient in an injectable fibrin matrix after 2 weeks of in vitro cell culture
applicated cells are capable of dividing with high growth potential, form growing epidermal islands in wound bed

3. Primary culture of epidermal cells after enzymatic dissociation

5. Posibilities of our method utilization
This skin-replacement product is designed to meet the needs mainly of the chronic ulcers and burns care

6. Keratinocytes cell suspension
3 weeks after
Keratinocytes cell suspension
in fibrin-glue
cells
transplantation
3 months after

10. Technology advantages
no rejection by patient – permanent wound covering
no adverse reactions in the clinic
no associated infections
no risk of disease transmission
significantly better wound closure efficancy in non-healing wounds than by using conventional noninvasive methods
scarless wound healing

11. Other skin-repacement matherials
Split-skin autotransplantation (require substantially large skin flaps – under general anesthesis)
Allografts or other biological dressing - temporal wound covering

12. Current status
development of strategic alliances for the promotion of the product
searching for substantial funding for the venture