1. Size Exclusion Chromatography *
Purification of nucleic acids and proteins
Desalting
Buffer exchange
Dye terminator removal
* = Gel Filtration

2. Size Exclusion Chromatography
Principle:
1. larger molecules pass the matrix faster
2. small molecules, which can enter the pores of the matrix particles, migrate slowly

3. Size Exclusion Chromatography
column
preparation
2. column
equilibration
3. sample
application
Volumes available:
1 ml, 200 µl, 15 µl
[96 well plates]
4. elute
fractions

4. Size Exclusion Chromatography
Excellent performance of DextraSEC Columns
Comparison of e.g. the removal of ammonia from oligonucleotides
NAP 10 Column (GE Healthcare)
DextraSEC NA10 AppliChem
Higher purity within a smaller
elution volume

5. Size Exclusion Chromatography
AppliChem's products available:
AppliXchange-G25 M
DextraSEC Columns
DextraSEC Plates

6. Size Exclusion Chromatography
AppliChem's sales arguments:
over 90 % recovery
high chemical stability
high resolution
easy-to-use
best price – best value for money

7. Size Exclusion Chromatography
A Survey of the market: (1) Matrix substances
Supplier
Matrix
Amount
Price*
AppliChem
Dextran-based matrix:
AppliXchange G25 M
100 g
€ 192,60
Sepharose-based matrix Illustra
Sephadex G-25 GE
Healthcare
100 g
€ 203,-
BioRad
Polyacrylamide-based matrix
Bio-Gel P-6DG Gel
100 g
€ 269,-
*: acc. to Euro price lists 2009/10

8. Size Exclusion Chromatography
A Survey of the market: (2) ready-to-use columns
Supplier
Name
Amount
Price*
AppliChem
DextraSEC NA10
50 columns
€ 255,- GE
Healthcare
illustra NAP 10
50 columns
€ 299,-
Econo-Pac 10DG
30 columns
€ 340,-
BioRad
*: acc. to Euro price lists 2009/10

9. Size Exclusion Chromatography
Literature