1. Microscope Basics Parts and focusing

2. Label the Compound Light Microscope (Handout) Ocular lens Body Tube Coarse Adjustment Knob Fine adjustment Knob Revolving nosepiece Objective lenses (4x, 10x, 40x) ArmStage Stage Clips Diaphram Light source Base

3. Focusing with a Microscope Plug in and turn on. Plug in and turn on. Lower stage completely. Lower stage completely. Turn nosepiece to low objective. Turn nosepiece to low objective. Raise stage with coarse focus knob until specimen is seen. (Big knob) Look from the side. Raise stage with coarse focus knob until specimen is seen. (Big knob) Look from the side. Fine focus the specimen. (Smaller knob) Fine focus the specimen. (Smaller knob) Centre specimen in the field of view. Centre specimen in the field of view.

4. Rotate nosepiece to medium power objective. Look from the side. Rotate nosepiece to medium power objective. Look from the side. Refocus with fine adjustment. Refocus with fine adjustment. Re-centre and focus using the fine adjustment. Re-centre and focus using the fine adjustment. Adjust the diaphragm. (Light) Adjust the diaphragm. (Light) Start over to look at another specimen. Start over to look at another specimen. Carrying and storage instructions. Carrying and storage instructions.

5. Microscope and Cell Theory Assignment – pages 141 Microscope and Cell Theory Assignment – pages 141 Questions 1 & 4 in green box Questions 1 & 4 in green box