1. Chem 217 Metallobiochemistry T/Th 8-9:20AM, W 12-12:50 Pat Farmer PSI 319 x6079, pfarmer@uci.edu Office hours: – MW 8-9AM and by appointment

2. Overview Emphasis will be on the structural motifs of metalloprotein centers and their associated reactivities. Course material will offer broad exposure to multidisciplinary topics and methodologies. Prerequisites include undergraduate inorganic chemistry, physical or analytical chemistry, some knowledge of biochemistry. The major goal of this course is to provide you with the background and tools necessary for a critical appraisal of current bioinorganic topics in the literature. One research project will be required, given as a 3-5 page report at the end of the term.

3. Understanding Protein Structure 4 Looking at Protein Images –what do those squiggles mean 4 Downloading Structures from the PDB –something you can do through out the course 4 Imaging an Active Site in the Modeling Facility –make an image and see it on the web

4. Bioinorganic Motifs Hemeproteins, – the colors of life FeS clusters, – from a time before oxygen Cu proteins, – versatile redox centers Zn, Ca – signal transducers

5. Heme Proteins: The colors of life –“ Why the bloode is red and the grasse green, these are mysteries no Man can fathom” John Dunne 17xx? 4 heme are ubiquitous –one structure/many functions –function controled by oxidation state ligand binding

6. FeS clusters: an ancient motif 4 ubiquitous in mitochondria 4 self-assembly of clusters 4 optimized for 4 fast electron transfer –minimum reorganization –electron delocalization

7. Cu Proteins: 4 Controlled Redox –oxygen binding and activation – cyt c oxidase, THE ultimate electron acceptor 4 Radical Generation –tyrosinase

8. Signal Transduction 4 Ca binding proteins –EF hand motif intercellular signal amplification 4 Zn binding proteins –Zn fingers –DNA binding gene regulation

9. Procedures 4 Highlight any procedural differences from regular projects of this type 4 Discuss requirements, benefits, and issues of using new procedures List location or contact for procedures document (or other related documents) here FOR MORE INFO...

10. Schedule 4 Review high-level schedule milestones here List location or contact for detailed schedule (or other related documents) here FOR MORE INFO...

11. Current Status 4 High-level overview of progress against schedule –On-track in what areas –Behind in what areas –Ahead in what areas 4 Unexpected delays or issues

12. Related Documents 4 marketing plan –location or contact name/phone 4 budget –location or contact name/phone 4 post mortem –location or contact name/phone 4 submit questions –location or contact name/phone