1. Homework 3 1.Plot the gene expression profile of the assigned 4 genes from NCI60. 2.Intersect the same genes from SOURCE and protein-protein interactions.

2. click

4. Click color bar

5. Similar expression genes

6. Download raw data

7. 1. Download raw data 2. The data will be a.zip file. 3. There will be 68 separate files in there and will be difficult to retrieve your genes 68 times.

8. To obtain a single “cleaned” file, you need to use the following steps. Cleaned file=normalized and retrieved file.

9. Select

10. Find the NCI60 article

11. Select

13. 1. Select all 3. Select 2. Select

14. 1. Cancel the selection

15. Wait for results. It may take more than 4 hrs. Here are different steps for data retrieval.

16. Select

17. 1. Select all data 2. Open a new excel file and paste 3. See next page

18. 1.Paste to Excel 2.Find genes (ie. stk6) from column B Name in excel 用 ‘ 編輯 ’ 選項中 ‘ 尋找 ’ 功 能 3.Plot the figure

19. Please plot your assigned genes by Excel

20. TACC3 Hec Nuf2  CENP-A  TPX2   HURP LymphomaCarcinoma classification NCI60Normal Tissue Atlas HeLa Cell cycle database substrate http://source.stanford.edu