1. 천연물 유래 암줄기세포 억제제 분석 시스템 구축 제주대학교 아열대 원예 산업연구소 최학선

2. I. Definition of cancer stem cells II. Markers of cancer stem cells III. Model of caner stem cells VI: Relationship between metastasis and cancer stem cells Contents

3. Minimal Definition of Cancer Stem Cells 1)Distinct markers allowing purification 2)A high capacity to form tumors as compared to other tumor cells 3) Ability to differentiate and recreate all cell phenotypes of the parental tumor 4) Self-renewal, tumor sphere formation in low-adherence cultures, and multi-drug resistance

4. Tumor TypeMarkers for CSC population Brain TumorsCD133 + Breast CancerCD24 -low /CD44 +high /ESA + Ovarian CancerCD133 + /Side population (SP)/CD44 +, CD117 + Lung CancerCD133 + Prostate CancerCD44 + /α2β1 high /CD133 + Pancreatic CancerCD44 + /CD24 + /ESA/CD133 + Hepatocellular CancerCD133 + Hematological MalignanciesCD34 + /CD38 - Colon CancerCD133 + /CD44 + /Lin - /ESA + Head and Neck CancerCD44 + Table 1. 암줄기세포 마커

5. Aldehyde dehydrogenase 형광물질 기질을 사용하여 ALDH positive cell 검색기술 Cancer stem cell assay using ALDEFLUOR assay : separation of aldehyde dehydrogenase-positive population by FACS

6. Breast cancer cell lines contain functional cancer stem cells with metastatic capacity and a distinct molecular signature [Cancer Res. 2009; 69(4): 1302–1313]

7. Models of cancer stem cells formation Frontier in physiology 2013(4)225:1-10

8. Metastatic cascade Seminars in Cancer biology 2012 (22) 396-403

9. Cancer stem cell necessity in metastasis Tumor Biol. (2013) 34:2019–2030

10. A B MCF-7 Mammospheres Figure 1. Formation of mammospheres C 10 0 10 2 10 1 10 4 20 40 60 80 100 120 10 3 0 % of Max MCF-7 Mammosphere

11. H Figure 2. The automated counting of mammospheres using the NICE software NICE analysis Mammosphere culture D E F G Original Image Histogram Fitting Threshold Counts Original Counted MFE (%) MCF-7 Mammosphere formation assay

12. Figure 3. Effect of metformin on mammosphere formation efficiency MCF-7 cells were exposed to mammosphere culture media with metformin for seven days. A B C MFE (Relative to vehicle %) * * 0 0055 5 10 mM Metformin Original imageCounts using NICEMammosphere Assay 0 0 10 0 0 10 mM Metformin D