2. Transcription Definitely not Transformation, Transduction or Translocation!

3. What is a Gene? One Gene – One Enzyme One Gene – One Polypeptide One Gene – One RNA transcript A GENE IS A SEGMENT OF DNA THAT CODES FOR - - - - - - - - AN RNA TRANSCRIPT!!!!

4. GenerRNA Component of Ribosome tRNA Carries amino acids to Ribosome mRNA Codes for Polypeptide snRNA Helps to splice primary transcripts miRNA Helps regulate gene expression

5. Which of the following will get translated? A.rRNA B.tRNA C.mRNA D.snRNA E.miRNA 1.A only 2.B only 3.C only 4.D only 5.E only 6.A & C 7.D & E 8.A, B, & C 9.A, B, C, D, & E 1234567891011121314151617181920 212223242526

6. Which of the following are directly involved with translation? A.rRNA B.tRNA C.mRNA D.snRNA E.miRNA 1.A only 2.B only 3.C only 4.D only 5.E only 6.A, C, & E 7.B & C 8.A, B, & C 9.A, B, C, & D 1234567891011121314151617181920 212223242526

7. What best represents rRNA? 1.Red 2.Blue 3.Green 4.Brown 1234567891011121314151617181920 212223242526

8. What best represents tRNA? 1.Red 2.Blue 3.Green 4.Brown 1234567891011121314151617181920 212223242526

9. What best represents snRNA? 1.Red 2.Blue 3.Green 4.Brown 1234567891011121314151617181920 212223242526

10. Life is just an RNA SAMWICHH!

11. Hey Check out this basic transcription animation! http://www- class.unl.edu/biochem/gp2/m_biology/animat ion/gene/gene_a2.htm http://www- class.unl.edu/biochem/gp2/m_biology/animat ion/gene/gene_a2.htm l

12. What did the idiot scribe represent? 1.RNA primase 2.RNA polymerase 3.DNA polymerase 4.Reverse transcriptase 5.Helicase

13. What is/are the substrate (s) for RNA polymerase? 1.ATP, GTP, UTP, CTP 2.ATP only 3. d ATP, d GTP, d CTP, d TTP 4.ATP, GTP, CTP, TTP 5.Amino acids

14. How many RNA polymerases are there? THREE RNA Polymerase I – Transcribes nucleolar genes into rRNA RNA Polymerase II – Transcribes genes into mRNA (which eventually turns into a polypeptide) RNA Polymerase III – Transcribes genes into tRNA, snRNA, & miRNA

15. How does an RNA Polymerase “FIND” a gene to transcribe? TRANSCRIPTION FACTORS FIND A TATA BOX ON THE PROMOTER REGION!!!!!!!!!!!!!!!!!!!!

17. 3’ GGGCCCACGGAGCACAAAAA TTTTT ATATTTTT ATAGGGCCCGAGGTGTACGTCGAGCCC TTTACCATT GCGGGGAACACATTC TTATTTCCC GCGGACCC ACATCA TCAGGGG 5’ 5’ CCCGGGTGCCTCGTGTTTTTTAAAAATATAAAAATATCCCGGG CTC CACATGCAGCTCGGGAAATGGTAACGCCCC TTGTGTAAGAATAAAGGGCGCCTGGGTGTAGTAGTCCCC 3’ 3’ TACGTCGAGCCC TTTACCATT GCGGGGAACACATTC TTATTTCCC GCGGACCC ACATCA TCAGGGG 5’

21. Check Out the Process in a MovEEE http://vcell.ndsu.edu/animations/transcriptio n/index.htm http://vcell.ndsu.edu/animations/transcriptio n/index.htm

22. What did Sir Spliceolot accomplish? 1.Cut out different DNA sections called introns. 2.Cut out different RNA sections called exons. 3.Cut out different RNA sections called introns. 4.Cut out different DNA sections called exons.

23. Why are introns called introns? They are INTERVENING noncoding sequences found within a gene!

24. Why are exons called exons? They are coding sections of a gene that get EXPRESSED as a component of a protein!

25. What really splices out the introns? 1.Cutterase 2.Dicerase 3.Spliceosome 4.snRNPase 5.Slicease 1234567891011121314151617181920 212223242526

26. What really is a SPLICEOSOME? snRNA small protein BIG PROTEIN snRNP SPLICEOSOME