1. PURE PROJECT TURKEY Aytekin Oğuz - Neşe İmeryüz

2. Sampling Frame & Recruitment UrbanRural Definition Population size >20.000 Population size ≤ 20.000 ≤ 20.000 Location Istanbul –Northwest Ankara – Middle Anatolia Adana – South Anatolia Samsun – North Anatolia Erzurum – East Anatolia Settlements within 2-hour driving distance but at least 50 km away from the urban cities

3. Definition of rural: Population size ≤ 20.000 Selection of rural settlements: Villages within 2-hour driving distance but at least 50 km away from the urban cities

4. Sampling Frame & Recruitment UrbanRural Facilitating organisation The major teaching hospital located within the urban city Muhtar, the official administrative person of the neighbourhood and health care providers within the settlement Sampling frame Feasiblity for long term follow-up The rural settlements that have a relatively stable population are selected, however rural to urban migration may be a considerable problem during the next 10 years

5. Sampling Frame & Recruitment UrbanRural Determine the percentage of population between the ages 35-70 2005 projection of 2000 cencus data (National Statistics Institute) is used The sampling method used is cluster sampling Clusters are selected from the list of streets (National Statistics Institute) 2005 projection of 2000 cencus data (National Statistics Institute) is used

6. Sampling Frame & Recruitment Urban & Rural Strafication of the population Choosing cities from the different geographical regions of Turkey provides a balanced selection regarding SES Choosing sample sizes The number of people selected in Istanbul 5000 * (the adult population of western 5000 * (the adult population of western Turkey / the total adult population of Turkey / the total adult population of Turkey) Turkey) The rural sample size of Istanbul = the total number of the subjects included from Istanbul * (the rural adult population from Istanbul * (the rural adult population of western Turkey / the urban adult population of western Turkey)

7. Population Sampling no of participant per study center LocalizationUrbanRural Istanbul1300500 Ankara650250 Samsun350350 Adana400300 Erzurum500400 Total number 32001800

8. Population Sampling No of clusters per study center Localization Urban cluster n Rural cluster n Istanbul265 Ankara132.5 Samsun73.5 Adana83 Erzurum104 Total number 6418 Urban cluster size = 50 people, rural cluster size = 100 people

9. Population Sampling No of families per study center Localization Number of urban families Number of rural families Istanbul325125 Ankara162.562.5 Samsun17587.5 Adana175100 Erzurum225125 Total number 1250800

10. Sampling Frame & Recruitment Urban & Rural Approaches to recruitment Door to door Site of data collection and initial blood processing Anthropometry, ECG, spirometry, grip- strength e.t.c. measurements will be done at the local health center. Blood and urine samples are taken at home or in the local hospital.

11. Steps taken until now Ethic Committee approval Done Done If genetic material will be kept in Turkey If genetic material will be kept in Turkey To export the genetic material we need approval of Central Ethics Committee of the Ministry of Health To export the genetic material we need approval of Central Ethics Committee of the Ministry of Health Any possibility to do genetic studies within Turkey? Any possibility to do genetic studies within Turkey?

12. Steps taken until now Application for a grant from TUBİTAK Declined Declined We are waiting for the reasons on which their decline was based We are waiting for the reasons on which their decline was based We are going to reapply after correcting shortcomings We are going to reapply after correcting shortcomings Re-evaluation of the project takes almost 3-4 months Re-evaluation of the project takes almost 3-4 months

13. Steps taken until now Semiquantitative FFQ A pilot study to prepare a semiquantitative food frequency questionnaire was designed and approved for financial support by TUBITAK but not started A pilot study to prepare a semiquantitative food frequency questionnaire was designed and approved for financial support by TUBITAK but not started

14. Steps ahead Translation and validation of questionnaires Translation and validation of questionnaires Meeting of the local committees Meeting of the local committees Recruitment and education of the surveying people, nurses, dieticians and laboratory technicians Recruitment and education of the surveying people, nurses, dieticians and laboratory technicians Standardization of the laboratories that will process the collected blood Standardization of the laboratories that will process the collected blood

15. People recruited to the study None None

16. Waiting for your suggestions to realize the project

17. Thank you very much for your attention