1. Log in using your Open Biosystems User Account Information and Partner Password Supplied by SIDNET * To create an Open Biosystems Account, log onto www.openbiosystems.com, click on “Login”, then follow the “Not a registered user? Click here to sign up.” Complete the application with correct and current contact information. Return to the SIDNET- Open Biosystems Portal to log in. www.openbiosystems.comhere If you have an existing Open Biosystems Account *, log onto the SIDNET-Open Biosystems Portal (http://sidnet.openbiosystems.com)http://sidnet.openbiosystems.com

2. To search the SIDNET shRNA collection select Human or Mouse GIPZ

3. Enter search term (Gene Symbol, Genbank Accession Number, Clone ID, etc.) i.e “Nedd4” To search for multiple genes enter search terms separated by commas (i.e NM_000123, BC001963) To order control vectors, ‘click’ the shopping cart next to your selection Using the main search box will search all Open Biosystems Inventory, not just SIDNET shRNA

4. Select the link for the # of clones available

5. “licensed” indicates clone is available from SIDNET Click on links to get detailed information on each shRNA construct

6. Clone specific details Select details to view, including sequence

7. Sequence information for sense, loop and antisense

8. 1. Click on shopping cart to add clone to your order 2. Click on “view cart” to check your order

9. Proceed to checkout

10. You must provide a cost centre # in the PO Number Field AND you must provide the Name of your Sick Kids PI in the Order/Quote Notes Field for your order to be processed!! Click “Place Order” to complete your transaction

11. When your order has been placed, your cart will be empty.

12. You will receive a confirmation email for your order. When your order is ready you will be contacted by SIDNET and provided with a Material Transfer Agreement to be signed by your PI to be brought with you to pick-up your clones.

13. Once you have logged into the SIDNET-Open Biosystems Portal, your Open Biosystems account will be linked to SIDNET, guaranteeing that as a Sick Kids Open Access member you will automatically get any shRNA in our collection at a significant discount. For all future orders, you can search only those shRNA clones that are at SIDNET by logging into the portal. Or, you can log into www.openbiosystems.com to search the entire Open Biosystems inventory. Any item that is available from SIDNET will have the price listed as “licensed”. When these items are placed in your cart, they will automatically be ordered directly from SIDNET.www.openbiosystems.com

14. To search all Open Biosystems Inventory, expand the search box, and enter your list of terms to search then hit submit 1. 2. 3.

15. Select the gene of interest from your list

16. Clones that are available at SIDNET will be listed as “licensed”, all other clones are available from Open Biosystems Proceed with your order by placing your clones of interest into your shopping cart as previously instructed.