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Temporary Presence of Myofibroblasts in Human Elbow Capsule After Trauma by Job N. Doornberg, Tjalling Bosse, Mark S. Cohen, Jesse B. Jupiter, David Ring,

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Presentation on theme: "Temporary Presence of Myofibroblasts in Human Elbow Capsule After Trauma by Job N. Doornberg, Tjalling Bosse, Mark S. Cohen, Jesse B. Jupiter, David Ring,"— Presentation transcript:

1 Temporary Presence of Myofibroblasts in Human Elbow Capsule After Trauma by Job N. Doornberg, Tjalling Bosse, Mark S. Cohen, Jesse B. Jupiter, David Ring, and Peter Kloen J Bone Joint Surg Am Volume 96(5):e36 March 5, 2014 ©2014 by The Journal of Bone and Joint Surgery, Inc.

2 Job N. Doornberg et al. J Bone Joint Surg Am 2014;96:e36 ©2014 by The Journal of Bone and Joint Surgery, Inc.

3 Job N. Doornberg et al. J Bone Joint Surg Am 2014;96:e36 ©2014 by The Journal of Bone and Joint Surgery, Inc.

4 Job N. Doornberg et al. J Bone Joint Surg Am 2014;96:e36 ©2014 by The Journal of Bone and Joint Surgery, Inc.

5 Acute trauma for less than seven days: a focus of organizing granulation tissue with extravasation of red cells, influx of macrophages, and inconspicuous surface synovium. Job N. Doornberg et al. J Bone Joint Surg Am 2014;96:e36 ©2014 by The Journal of Bone and Joint Surgery, Inc.

6 Staining for α-SMA revealed no expression of α-SMA in fibroblast-like cells; of note, smooth muscle cells located in perivascular areas stain for α-SMA indicating specificity of staining (25×). Job N. Doornberg et al. J Bone Joint Surg Am 2014;96:e36 ©2014 by The Journal of Bone and Joint Surgery, Inc.

7 Job N. Doornberg et al. J Bone Joint Surg Am 2014;96:e36 ©2014 by The Journal of Bone and Joint Surgery, Inc.

8 Job N. Doornberg et al. J Bone Joint Surg Am 2014;96:e36 ©2014 by The Journal of Bone and Joint Surgery, Inc.

9 Job N. Doornberg et al. J Bone Joint Surg Am 2014;96:e36 ©2014 by The Journal of Bone and Joint Surgery, Inc.

10 Posttraumatic elbow contracture: fibrosis with increased dense plump collagen matrix with fibroblasts and no neovascularization (200×). Job N. Doornberg et al. J Bone Joint Surg Am 2014;96:e36 ©2014 by The Journal of Bone and Joint Surgery, Inc.

11 Staining for α-SMA revealed no expression of α-SMA in fibroblast-like cells; of note, smooth muscle cells located in perivascular areas did stain for α-SMA, indicating that our staining protocol for α-SMA was specific (200×). Job N. Doornberg et al. J Bone Joint Surg Am 2014;96:e36 ©2014 by The Journal of Bone and Joint Surgery, Inc.

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