1. Vicki Wysocki (University of Arizona) Probing Influence of Charge on Structures of Protein Complexes with Surface Induced Dissociation and Ion Mobility Bela Paizs (German Cancer Research Center, Heidelberg) Efficient Computation of Structures and Reactions of Doubly Protonated Tryptic Peptides and Their Fragments Julia Laskin (Pacific Northwest National Laboratory) Large Metal and Metal-Oxide Clusters in the Gas Phase and on Surfaces Ryan Julian (University of California, Riverside) Radical Recombination Labeling: Click Chemistry in the Gas Phase Oliver Hampe (Karlsruhe Institute of Technology) Multiply Charged Anions: Infrared Spectroscopy and Photoelectron Spectroscopy on Adenonsine 5'-Di and Triphosphate Anions Ion Activation and Dissociation

2. What are the means of ion activation? Collisions with gases: Collision-induced dissociation (CID) Reactions Collisions with electrons: Electron capture dissociation (ECD) Electron ionization Collisions with other ions: Electron transfer dissociation (ETD) Collisions with surfaces: Surface induced dissociation (SID) Collisions with photons: Photoionization – PEPICO Infrared – IRMPD, tagging, BIRD UV-visible

3. Ion Activation and Dissociation What factors are influential? Structure (conformation) of initial ion Extent and distribution of energy deposited Timescale of experiment (lifetimes) Mechanism of excitation (rovibronic vs electronic) What do we learn? Structure Energetics Kinetics Reactivity

4. Structural and Energetic Information Means of generating ions changes structure More, Ray, Armentrout, J. Phys. Chem. A 1997, 101, 7007. Armentrout, Austin, Rodgers, Int. J. Mass Spectrom. 2012, 330-332, 16. Thermodynamic Kinetic trapping

5. Structural and Energetic Information Chen, Rodgers, J. Am. Chem. Soc. 2012, 134, 5863 Molecular Recognition of Amino Acids by 18-Crown-6 Binding energy (Gly)H + -18C6 (N-terminus) > (Lys)H + -18C6 > (His)H + -18C6 > (Arg)H + -18C6

6. Donald, Williams, Pure Appl. Chem. 2011, 83, 2129 Electron capture dissociation of M 3+ (NH 3 ) 6 (H 2 O) 55 Also UV photodissociation of M 2+ (H 2 O) n Energetic Information - Electrochemistry Relative solution phase Absolute gas phase

7. Energetic Information – Biochemistry DNA 4.1 ± 4.3 RNA 6.5 ± 8.6 MAD ± SD Energy required for glycosidic bond cleavage: DNA < RNA Glycosidic bond cleavage of nucleosides and deoxynucleosides Protonated deoxyadenosine [Ado+H + ] N3, syn, C 2'-endo Wu and Rodgers, work in progress

8. Vicki Wysocki (University of Arizona) Probing Influence of Charge on Structures of Protein Complexes with Surface Induced Dissociation and Ion Mobility Bela Paizs (German Cancer Research Center, Heidelberg) Efficient Computation of Structures and Reactions of Doubly Protonated Tryptic Peptides and Their Fragments Julia Laskin (Pacific Northwest National Laboratory) Large Metal and Metal-Oxide Clusters in the Gas Phase and on Surfaces Ryan Julian (University of California, Riverside) Radical Recombination Labeling: Click Chemistry in the Gas Phase Oliver Hampe (Karlsruhe Institute of Technology) Multiply Charged Anions: Infrared Spectroscopy and Photoelectron Spectroscopy on Adenonsine 5'-Di and Triphosphate Anions Ion Activation and Dissociation