1. TEIN 4: RNA-seq analysis Akzam Saidin Sr. Bioinformatics Scientist

2. RNA Transcription and Processing

3. Gene Structure and Alternative Splicing

4. 2015 NOVOCRAFT TECHNOLOGIES. PROPRIETARY AND CONFIDENTIAL.

12. Alignment Accuracy Trial 1Trial 2

14. Figure from Wang et. al, RNA-Seq: a revolutionary tool for transcriptomics, Nat. Rev. Genetics 10, 57-63, 2009.

15. 2015 NOVOCRAFT TECHNOLOGIES. PROPRIETARY AND CONFIDENTIAL.

17. References 1. KR Kukurba and SB Montgomery. RNA Sequencing and Analysis. 2015 Cold Spring Harb Protoc; doi:10.1101/pdb.top084970 2. M Griffith, JR Walker, NC Spies, BJ Ainscough, OL Griffith. Informatics for RNA Sequencing: A Web Resource for Analysis on the Cloud. 2015 PLOS Computational Biology. DOI: 10.1371/journal.pcbi.1004393 3. Z Wang, M Gerstein and M Snyder. RNA-Seq: a revolutionary tool for transcriptomics. 2009 Nat Rev Genet. 10(1):57-63. doi: 10.1038/nrg2484 2015 NOVOCRAFT TECHNOLOGIES. PROPRIETARY AND CONFIDENTIAL.