1. Drosophila Identification of various phenotypes.
Biology 22
Drosophila
Identification of various phenotypes.

2. Reference for Pictures:
Carolina Drosophila melanogster Manual ( )
Published: Carolina Biological Supply Company

3. The life Cycle

4. Life Cycle: Drosophila melanogaster
Eggs: contain filaments
2 molting phases “instars”
Life cycle is 10 days

5. Fruity Fly Egg and Larva

6. Fruit Fly Pupa and Adult

7. Identification of:
Gender
Body Color

8. Wild Type Versus Mutants
Body Color
Wild type: Beige
Mutant: Yellow body

9. Parental phenotypes Experiment: body color
A= female: wt (beige)
B=female: wt (beige)
C=male: wt (beige)
D=female: mutant (yellow)
E=male: mutant (yellow)

10. Fruit Fly Body Color

11. Sex combs: Male Drosophila

12. Abdominal segements 7 segments to female abdominal segments
5 segments to male abdominal segments
Male body size is smaller than female
But the “last” abdominal segment is larger in male

13. Nap Time Triethylamine

14. How to anesthetize a fly

15. How to use the Napper

16. View the Fruit Fly

17. You will record class data
After counting the crosses record the data on the class spreadsheet

19. 3 point test cross: Experiment 2
Eye color
Body color
Wing length

20. 3 point test cross for: Beige (bl+) vs. Black (bl) 1. Body Color
♦ Wt vs Mutant
Beige (bl+) vs. Black (bl)
Long(vg+) vs. Vestigal(vg)
Brick red (br+) vs. brown (br)
1. Body Color
2. Wing length
3. Eye Color

21. Crosses:
Female Males
Males and female progeny from this F2 cross are your 3 point test cross results!
bl+ vg+ Bw+
bl vg bw X
bl vg bw
bl vg bw

22. There are all sorts of eyes!

23. Eyes

24. You will have only red eyes (wt)

25. Fruit Fly Wings

29. Parental phenotypes A= female: wt beige body B=female: wt beige body
C=male: wt beige body
D=female: yellow body
E=male: yellow body

30. Female and Male Fruit Flies