1. DIET INFLUENCE IN PROTEIN AND FATTY ACID CONTENT OF BREAST MILK FROM DIFFERENT ORIGIN Rubén López Nicolás rubenln@um.es FATTY ACID PROTEIN

2. INTRODUCTION HUMAN MILK IS THE OPTIMAL MODE OF INFANT FEEDING THE BENEFITS TO INFECTIOUS GASTROINTESTINAL DISEASE BIOACTIVE PROTEINS LONG-CHAIN FATTY ACIDS Wada Y., Lönnerdal B. (2014) Bioactive peptides derived from human milk proteins – mechanisms of action. The Journal of Nutritional Biochemistry INTRODUCTIONOBJECTIVESMATERIAL AND METHODSRESULTSCONCLUSIONS 1/12

3. INTRODUCTION BIOACTIVE PROTEINS LACTOFERRIN (LF) HAPTOCORRIN (HC) 2/12 LONG-CHAIN FATTY ACIDS ARACHIDIC ACID LINOLELAIDIC ACID ELAIDIC ACID CIS-10-HEPTADECANOIC ACID PALMITOLEIC ACID MYRISTOLEI ACID TRIDECANOIC ACID CAPRYLIC ACID INTRODUCTIONOBJECTIVESMATERIAL AND METHODSRESULTSCONCLUSIONS

4. 3/12 OBJECTIVESMATERIAL AND METHODSRESULTSCONCLUSIONS Study the effect of maternal diet and geographical localization in the protein and fatty acid composition in breast milk. SPECIFIC OBJECTIVES: The determination of Lactoferrin content in breast milk from women from Honduras, Egypt and Spain. The determination of Haptocorrin content in breast milk from women from Honduras, Egypt and Spain. The determination of long-chain fatty acid content in breast milk from women from Honduras, Egypt and Spain. The comparison of protein and fatty acid profiles of human milk samples from each origin.

5. 4/12 MATERIAL AND METHODS RESULTSCONCLUSIONS Breast milk samples were analyzed from each point of origin: 8 samples from Egypt, 7 samples from Honduras and 2 samples from Spain. These samples were taken during the 2 nd to 4 th month after birth Lactoferrin and Haptocorrin analysis Fatty Acid Extraction and Analysis

6. MATERIAL AND METHODSRESULTSCONCLUSIONS MATERIAL AND METHODS 5/12

7. MATERIAL AND METHODSRESULTSCONCLUSIONS Diagram of the electrophoresis of the Western Blot procedure MATERIAL AND METHODS WETERN BLOT Diagram of the antibody exposion phase of the Western Blot procedure 6/12

8. 7/12 MATERIAL AND METHODSRESULTSCONCLUSIONS MATERIAL AND METHODS FATTY ACID EXTRACTION AND ANALYSIS Or-Rashid M.M., Fisher R., Karrow N., AlZahal O., McBride B.W. (2010) Fatty acid profile of colostrum and milk of ewes supplemented with fish meal and the subsequent plasma fatty acid status of their lambs. Journal of Animal Science

9. RESULTSCONCLUSIONS 8/12 RESULTS LACTOFERRIN AND HAPTOCORRIN Lactoferrin Haptocorrin

10. RESULTS CONCLUSIONS LACTOFERRIN HAPTOCORRIN 9/12 Figure 2.Percentage of HC in human breast milk from different countries Figure 1.Percentage of LF in human breast milk from different countries

11. RESULTS CONCLUSIONS LONG-CHAIN FATTY ACIDS 10/12

12. RESULTSCONCLUSIONS LONG-CHAIN FATTY ACIDS RESULTS Figure 3. Relative percentage of LCFA from Spain, Honduras and Egypt 11/12

13. 12/12 CONCLUSIONS RESULTS The differences observed in the three countries could perfectly be because of the different diets consumed in each country. Infants in Spain ingest more LF so that could explain a lower incidence in gastrointestinal infections, lower anemia rates, etc. Infants in Egypt and Honduras consume more HC, so that would involve a better neural development and lower megaloblastic anemia rates. Infants from all three countries have a high consumption of arachidic acid which would help with the development of the nervous system and the immune system.

14. ACKNOWLEDGEMENTS Department of Food Technology, Nutrition and Food Science, Faculty of Veterinary Medicine University of Murcia

15. THANK YOU SO MUCH