1. Food preservation by drying

2. Fish drying Small fishes - dried whole; large fish - dried by cutting open or cutting to small pieces to ensure faster drying Fish may be salted or not salted before drying After drying, fish are packed and stored for later consumption as human food The storage stability or shelflife of dried fish depends on - quality of the raw material used, extent of drying and storage conditions to which fish is exposed after drying

3. Categories of dried foods Dried foods are generally divided in to 2 categories based on moisture content- Low moisture foods: These foods do not contain moisture level more than 25%, and have a w of 0.6 or less. These food have better keeping quality and shelflife Intermediate moisture foods (IMF): These contain 15-50% moisture and a w between 0.60 and 0.85.Ex: Dried fruits, cakes etc.

4. Effects of drying on microorganisms: Drying reduces the microbial activity in foods to a large extent, and depends on the moisture level attained after drying The storage stability (spoilage) of dried foods depends mainly on the a w of food Bacteria require high a w for growth (> 0.90) compared to yeasts and molds At a w. of 8.0~0.85: Spoilage of dried foods is caused by a variety of fungi and spoilage occurs in 1-2 weeks period. At a w of 0.75: Delayed spoilage caused by few types of organisms (fungi). At a w of 0.70: Spoilage greatly delayed, may not occur during prolonged storage. At a w of 0.65: Very few organism can grow, and spoilage is unlikely for 2 years.

5. Growth of most organisms is prevented at a w <0.65, but some molds (Ex: Aspergillus echinulatus, Zygosaccharomyces rouxii) are able to grow and cause spoilage Molds involved in the spoilage of dried foods are Candida, Botrytis, Rhizopus, Mucor, Saccharomycopsis, Alternaria, Aspergillus, Zygosaccharomyces etc. Endospores of bacteria, yeasts, molds and certain bacteria survive drying process Microbial spoilage of dried foods - prevented by storing at low Rh condition Storage at high Rh condition enables absorption of moisture from the atmosphere by dried foods until equilibrium is reached leading to spoilage beginning from the product surface

6. Microorganisms associated with dried fish: Spoilage of dried fish during storage can result from bacteria and fungi. Bacterial spoilage: Bacteria growing in salt dried fish (marine) are: - Slightly halophilic bacteria: These grow at 2~5 % salt. Ex: Many marine bacteria. - Moderately halophilic bacteria: These grow at 5-20 % salt content. Ex: Many bacteria such as Pseudomonas, Vibrio, Moraxella, Micrococcus, Acinetobacter, Flavobacterium etc. - Exstermely halophilic bacteria: These tolerate 20-32 % salt. Ex: Halobacterium, Micrococcus, Sarcina etc. Exstremely halophilic bacteria cause intense red/pink pigmentation in dried food.

7. Halophic bacteria are capable of growing at low R H (75%) unlike other bacteria The main source of these bacteria to fish is through the salt used for salting of fish These grow on surface of salt dried fish resulting in red coloration / pigmentation

8. Fungal spoilage: Fungi are the common cause of spoilage of salt dried fish Being aerobic and requiring low a w these grow on surface Ex: Aspergillus, Penicillium, Fusarium, Paecilomyces etc