3. Laser Flow Cytometry

4. Forward Scatter indicates size Forward Scatter

5. Side Scatter indicates complexity Side Scatter

6. Fluorescent wavelength detector FITC labeled cell! Fluorescence Measurements

7. PE labeled cell! Fluorescence Measurements Fluorescent wavelength detector

8. Fluorescence Detection FL-1: FITC, CFSE, ALEXA FL-2: PE, PI FL-3: Texas Red, Red-670 FL-4: APC, Topro

9. This demonstrates gating by size and complexity based on forward scatter and side scatter, respectively. Size and Complexity Separation

10. Negative Control This sample is a negative control. It has no conjugated stains and represents the overall background. No Stain

11. CD4:FITC No Stain Single Staining This sample has a single stain of CD4 labelled with FITC stain, giving a green color to those cells expressing CD4.

12. No Stain CD8:PE Single Staining This sample has a single stain of CD8 labelled with PE stain, giving an orange color to those cells expressing CD8.

13. CD4:FITC CD8:PE Double Staining This sample has a double stain of CD4:FITC and CD8:PE, giving a green and orange color to those cells expressing both CD4 and CD8.