1. Entomology

2. Introduction o Entomology is the study of insects o Forensic entomology is the study of the insects associated with a dead body o Also known as medicolegal o Forensic entomologists are called into homicide investigations when time of death is unknown and their evidence is usually presented in court as expert testimony

3. Introduction o Insects begin colonizing at a dead body immediately after death o Dependent on season and environment o Rate of development of the species can be used to estimate time since death o Can give an estimate up to a year

4. Introduction o Entomological evidence is the most accurate and frequently the only method available to determine the elapsed time since death o Due to rigor, algor, and livor mortis being useless after 72 hours

5. Introduction o Insects are also used to o Determine whether the body has been moved after death o Determine whether it has been disturbed o Determine the presence or position of wound sites o Determine whether the victim used drugs or was poisoned o Determine the length of time of neglect or abuse in living victims

6. Importance of Determining Time Since Death o Important for the family of the deceased o Understanding how, when, and why a person has died can help to give closure to family and friends and allow them to move on with their lives

7. Importance of Determining Time Since Death o Timing of death may also have legal implications o Insurance policies o Whether death occurred before or after insurance coverage began o Important for families when money is involved

8. Importance of Determining Time Since Death o May indicate the length of time that a fraud has occurred o Receiving monies for family members while family members are deceased

9. Decomposition o Begins at the moment of death, caused by two factors o Autolysis o The breaking down of tissues by the body's own internal chemicals and enzymes o Putrefaction o The breakdown of tissues by bacteria o These processes release gases that are the chief source of the characteristic odor of dead bodies o These gases swell the body

10. Decomposition o Scavengers play an important role in decomposition o Insects and other animals are typically the next agent of decomposition, if the body is accessible to them o The most important insects that are typically involved in the process include the fleshflies (Sarcophagidae) and blowflies (Calliphoridae) o The green-bottle fly seen in the summer is a blowfly o Larger scavengers, including coyotes, dogs, wolves, foxes, rats, and mice may eat a body if it is accessible to them o Some of these animals also remove and scatter bones.

11. Factors Involved in Decomposition o In a roughly descending degree of importance, those factors include: o Temperature o The availability of oxygen o Prior embalming o Cause of death o Access by insects o Burial, and depth of burial o Access by scavengers o Trauma, including wounds and crushing blows o Humidity, or dryness o Rainfall o Body size and weight o Clothing o The surface on which the body rests

12. Determination of Elapsed Time Since Death o First method based on the predictable development of larval Diptera, known as the blow fly o Used from the first time the first egg is laid on the remains until the first adult flies emerge from the pupal cases and leave the body o Evidence valuable from a few hours to several weeks after death

13. Determination of Elapsed Time Since Death o Second method is based on the predictable, successional colonization of the body by a sequence of carrion insects o Can be used from a few weeks after death until nothing but dry bones remain

15. Blow Flies o Blow flies are the first flies to be attracted to a body o They are large, metallic flies seen near food or garbage cans in summer o Blow flies belong to the family Calliphoridae, in the order Diptera or “true flies”

17. Blow Flies o Male and female blow flies require a protein meal before the ovaries and testes develop and oogenesis and spermatogenesis can occur o Adult feeding may occur at the dead body o Majority of the flies attracted to remains are females searching for egg laying sites

19. Blow Flies o Blow flies develop from eggs through the first, second, and third instar stages, and then the pupal stage before becoming adults o Stages influenced by species of blow flies and temperature of surroundings

20. Blow Flies o Insects are cold-blooded so their development is temperature dependent o As temperature increases, they develop more rapidly o As temperature decreases, they develop more slowly

21. Blow Flies o Analysis of the oldest insect stage on they body, together with knowledge of the meteorological conditions at the scene, can be used to determine how long insects have been feeding on the body, and hence, how long the victim has been dead

23. First Instar Stage o Once blow fly eggs have been laid, they will hatch into first instar larvae o Larvae rely on protein for their meals o Females lay eggs on open wounds of dead individuals or around orifices of a living individual o Face is colonized before other areas because the skin is easier to penetrate o Except in the case of rape cases, flies attracted to genetalia

24. Second Instar Stage o First instar larvae shed larval cuticle and mouthparts when entering second instar stage o Is more capable of penetrating the skin than the first instar larvae o Does so with proteolytic enzymes

25. Third Instar Stage o Third instar stage begins with second instar larvae shedding its cuticle o Are called maggots o Maggots aggregate together in large masses o Can remove a large amount of tissue in a very short amount of time o After feeding, the maggots move on to a site where they can pupate o Remove outer cuticle so they can emerge as a fly

27. Time Span o Female lays 2,000 eggs in her lifetime o Once eggs are laid, they hatch between 12 and 48 hours o It takes 14 days for the fly to emerge from the pupal case

28. Factors Used to Determine Time Since Death o 4 factors must be taken into account o Oldest stage of blow fly associated with the body o Look at old pupal cases o Species of insects o Each species develop at different rates so each species of insects at the scene need to be collected o Temperature data o Must be able to determine temperature of crime scene for a period of time o Developmental data o Must know how fast or how slow the specific species develop

29. Determining Whether the Body has been Moved o Insects present on the deceased body that are not prevalent to the crime scene can indicate that the victim was murdered elsewhere and was dumped at another location

30. Presence and Position of Wounds o Insects are attracted first and foremost to wounds so the first instar larvae will have access to liquid protein for nutrition o Wound sites in individuals that have completely decomposed are shown by irregular or atypical insect colonization

31. Linking Suspect to Scene o Sometimes, criminals carry entomological evidence on them unknowingly o This evidence can place them at the crime scene by examining the life cycles of the insects

32. Drugs o Insects that feed on the body of individuals that have been poisoned can be examined to determine what type of drug or toxin the person was poisoned with o It is important to note that specific drugs either speed up or slow down larval development o Can influence entomologist’s final report

33. Collection of Entomological Evidence o Evidence should be collected by an entomologist o If not available, a police death investigator should collect the evidence o Different stages of larval growth should be collected and bagged separately o Sample of soil should also be collected from just outside the area marked by body fluids

34. Challenges to Forensic Entomology o 3 challenges exist o Temperature o Temperature of crime scene and the temperature that the insects have been exposed to us unknown o Season o Entomology is valuable only in spring, summer, and fall o Exclusion of insects o Insects are excluded based on condition of body

35. Collect Preserved Samples o ƒ Preserved samples include specimens placed directly into 80% (or greater) ethyl alcohol o ƒFly eggs, larvae (maggots), pupae, newly emerged adults, etc. o ƒ Adult beetles, beetle larvae, ants, etc. o ƒ Adult flies collected using a sweep net can be: 1) placed directly into 80% ethyl alcohol; 2) anesthetized using an insect kill jar and then placed into 80% ethyl alcohol; or 3) placed into labeled sandwich bags and kept frozen until delivery

36. Collect Live Samples o Collect live specimens of fly eggs, larvae, and pupae from the same areas as the preserved samples o ƒ Place approx. 50-75 larvae of representative sizes on a food medium (raw hamburger, beef liver) and wrap loosely in aluminum foil. Place the raw meat and larvae into a rearing container with ½ to 1“ of vermiculite. o ƒ BE AWARE of the potential of placing too many eggs or young larvae into a single container. If so, simply separated the live larvae into additional containers, label and feed as specified.

37. Collect Live Samples o ƒ Be sure that the container lid is ventilated with very small holes to avoid escaping larvae. o ƒ Be sure to collect the LARGEST fly larvae present (or most mature larvae). o ƒ Be sure to look for presence of fly pupae. Place pupae in a ventilated rearing container with vermiculite (no raw meat needed).

38. o https://www.southeastern.edu/a cad_research/depts/biol/pdf/fe_ sampling_handout2.pdf