2.  Accurately measure and transfer small amounts of liquid  Volumes commonly range from 0.5 – 2000  L (1/millionth of a liter aka 10 -6 )  2-20  L in lab today

4.  In a P20, the top number refers to 10’s of μl, the middle number refers to μl’s and the bottom number refers to 1/10ths of μl.

5.  Never draw liquid directly into shaft of the pipette – ALWAYS HAVE A YELLOW PLASTIC TIP IN PLACE  Firmly press the micropipette shaft straight down onto a plastic tip  When finished with the tip press the ejector button to remove the disposable tip

7.  There are two stops  Depress the plunger to the 1 st stop  With tip held vertically in liquid slowly allow plunger to rise back to the release position to draw to up correct volume  To dispense slowly depress to the 1 st stop, pause a second, depress to 2 nd stop – plunger is flush with top of shaft – withdraw the tip without releasing the plunger

9.  Place tip below the surface but not all the way to the bottom

10.  Using Micropipettes Set pipette volume only within the range specified for that micropipette.  Do not attempt to set a volume beyond the pipette's minimum or maximum values. This will damage the micrometer gears!

11.  When using a micropipette, first apply a tip. Forgetting to do this would ruin the precision piston that measures fluid volume.  Always keep a micropipette in a vertical position when there is fluid in the tip. Do not allow liquid to accidentally run back into the piston.

12.  Use your thumb to control the speed at which the plunger rises after taking up or ejecting fluid. Letting it snap back damages the piston!  There should be no snapping noises.

13.  http://www.youtube.com/watch?v=BDadjDRl -Vg&feature=related http://www.youtube.com/watch?v=BDadjDRl -Vg&feature=related  http://www.youtube.com/watch?v=h06rz8rc Zpw http://www.youtube.com/watch?v=h06rz8rc Zpw