1. North Carolina State University Oat Maker Development Peter Maloney Jeanette Lyerly Paul Murphy Gina Brown-Guedira USDA-ARS Crop Science Department 840 Method Rd, Unit 3 Raleigh, NC 27695

2. Winter Hardiness Project Goal: To develop lines with increased tolerance to cold and freezing temperatures and map Winter Hardiness Component Traits (WHCT) Preliminary Work –Screened known Oat RFLP and SSR markers in Fulghum x Norline RIP phenotyped for WHCT –Tested Wheat SSR primers for amplification in Oat Markers were randomly chosen and had a very low success rate One oat SSR developed through this screening is associated with a QTL for WHCT

3. Winter Hardiness Project SSR markers Collaborating with Dr. Joe Anderson’s lab at Purdue University to test newly developed SSR markers Evaluated primers generated by the Anderson lab on a panel of 11 different cultivars –Used the Licor 4300 Analysis System Those that were polymorphic for Fulghum x Norline RIP were then mapped onto the population –**See poster by Peter Maloney for more information**

4. Winter Hardiness Project SNP discovery Sequence PCR fragments First SNP developed into CAPS1 marker –See Linkage Group FN7 for location* Develop SNP markers from oat ESTs Herman et al. (2006) described regions in wheat associated with up or down-regulation during cold acclimation ESTs from these genes were used to develop markers that were screened for amplification in our panel of oat cultivars –Through this process 2 SNPs and 1 deletion were found with a 4% success rate (* See poster by Peter Maloney)

5. Future Plans Develop cDNA libraries using oats that have been exposed to sub-zero temperatures Develop RIL to study BYDV resistance in oats Associate markers with BYDV resistance for marker assisted selection Develop RIL to study Grain Protein Content Identify markers that can be used in marker assisted selection for Grain Protein