1. Benthic Macroinvertebrate Sampling

2. Sampling -WHAT All available invertebrate taxa in the entire 200 foot stream reach -WHERE All available habitats in sample area Riffles Runs Pools Along stream margins

3. Capturing Specimens Find larger substrates rocks, woody debris, leaf packs Place net with small mesh downstream Gently disturb substrate upstream Capture dislodged specimens in net Collect 30 minutes minimum

4. Specimens in Net Rinse net remove small particles Dump left over debris in white pan Rinse net into pan Catch smaller quicker organisms with pipette 1 Collect until no new taxa observed (minimum 30 minutes)

5. Filling out PHWH Form Record the presence and abundance For all taxa Record this on the HMFEI Scoring Sheet Record Family or Genus For mayfly, caddisfly, stonefly groups

6. Voucher Specimens What are they? a preserved specimen a basis for further study evidence of validity of collection and identification a part of a permanent collection

7. How to preserve Materials Jars Tags Pencil Fluid Methods Place specimen(s) in jar Fill out tag Place tag in jar Add fluid to top Close tightly

8. Information for Field Tags Date Collector’s Name County Township Stream Identification