1. DEPARTMENT OF PRIMARY INDUSTRY, FISHERIES AND MINES Otolith Microchemistry

2. DEPARTMENT OF PRIMARY INDUSTRY, FISHERIES AND MINES AUSTRALIAN INSTITUTE OF MARINE SCIENCE Pilot study: Define the spatial structure of black jewfish stocks Aim CHARLES DARWIN UNIVERSITY

3. DEPARTMENT OF PRIMARY INDUSTRY, FISHERIES AND MINES AUSTRALIAN INSTITUTE OF MARINE SCIENCE Objectives Determine the elemental composition of otoliths Compare the composition of otoliths from different sites AUSTRALIAN INSTITUTE OF MARINE SCIENCE CHARLES DARWIN UNIVERSITY

4. DEPARTMENT OF PRIMARY INDUSTRY, FISHERIES AND MINES AUSTRALIAN INSTITUTE OF MARINE SCIENCE General Methods Otoliths were gained from aging project samples A total of 60 otoliths were analysed; 20 from each of the 3 sample sites –Caution Point –Chambers Bay –Peron Islands AUSTRALIAN INSTITUTE OF MARINE SCIENCE CHARLES DARWIN UNIVERSITY

5. DEPARTMENT OF PRIMARY INDUSTRY, FISHERIES AND MINES AUSTRALIAN INSTITUTE OF MARINE SCIENCE Map of sample sites Channel Point DarwinChambers Bay Caution Point AUSTRALIAN INSTITUTE OF MARINE SCIENCE CHARLES DARWIN UNIVERSITY

6. DEPARTMENT OF PRIMARY INDUSTRY, FISHERIES AND MINES AUSTRALIAN INSTITUTE OF MARINE SCIENCE Otoliths were dissolved in acid Element concentration determined by ICPMS Compare difference between 3 sites AUSTRALIAN INSTITUTE OF MARINE SCIENCE CHARLES DARWIN UNIVERSITY

7. DEPARTMENT OF PRIMARY INDUSTRY, FISHERIES AND MINES AUSTRALIAN INSTITUTE OF MARINE SCIENCE Results Caution Point Chambers Bay Peron Islands Site Concentration (ppb) Manganese AUSTRALIAN INSTITUTE OF MARINE SCIENCE CHARLES DARWIN UNIVERSITY

8. DEPARTMENT OF PRIMARY INDUSTRY, FISHERIES AND MINES AUSTRALIAN INSTITUTE OF MARINE SCIENCE Results Caution Point Chambers Bay Peron Islands Site Concentration (ppb) Barium (137) AUSTRALIAN INSTITUTE OF MARINE SCIENCE CHARLES DARWIN UNIVERSITY

9. DEPARTMENT OF PRIMARY INDUSTRY, FISHERIES AND MINES AUSTRALIAN INSTITUTE OF MARINE SCIENCE Results Caution Point Chambers Bay Peron Islands Site Concentration (ppb) Barium (138) AUSTRALIAN INSTITUTE OF MARINE SCIENCE CHARLES DARWIN UNIVERSITY

10. DEPARTMENT OF PRIMARY INDUSTRY, FISHERIES AND MINES AUSTRALIAN INSTITUTE OF MARINE SCIENCE Discussion Otolith microchemistry can be used to determine spatial structure of black jewfish stocks Solution based ICPMS is a suitable technique AUSTRALIAN INSTITUTE OF MARINE SCIENCE CHARLES DARWIN UNIVERSITY

11. DEPARTMENT OF PRIMARY INDUSTRY, FISHERIES AND MINES AUSTRALIAN INSTITUTE OF MARINE SCIENCE Summary Work in progress Perform LA-ICPMS analysis Compare age/element relationship CHARLES DARWIN UNIVERSITY