1. AppliChem © 2010 1/34 There is another top address in Darmstadt! Immunoassay buffer Principle of Immunoassays patentdocs.typepad.com/.../implications_of.html Types of Immunoassays o ELISA o RIA o Western blotting o Specific antibodies are used for the detection of proteins/epitopes o EIA o Immuno histochemistry o Immuno PCR

2. AppliChem © 2010 2/34 There is another top address in Darmstadt! Immunoassay buffer so close: perfect and useless A: The perfect sandwich ELISA (1 st and 2 nd antibody only bind to the antigen (blue). BC DE A B – L: Possible, unwanted side effects

3. AppliChem © 2010 3/34 There is another top address in Darmstadt! Immunoassay buffer for assay optimization o Prevent unspecific binding to surface or proteins. o Reduce background. o Reduce interference (matrix effects and cross-reactivity). o Increase stability. o Increase reproducibility.

4. AppliChem © 2010 4/34 There is another top address in Darmstadt! Immunoassay buffer Blocking Buffer I o best blocking efficiency o optimal size distribution of modified casein fragments o Compatible with all types of immunoassays (with all detection methods)

5. AppliChem © 2010 5/34 There is another top address in Darmstadt! Immunoassay buffer CrossDown Buffer without...... with CrossDown Example 2: Dot blot Example 1: Western blot o reduces unspecific binding (= with unknown substance) o reduces matrix effects (= sum of all negative effects) o reduces cross-reactivity (= non-target binding with known subst.)

6. AppliChem © 2010 6/34 There is another top address in Darmstadt! Immunoassay buffer Example 3: ELISA false positive binding with PBS false positive binding with CrossDown CrossDown Buffer

7. AppliChem © 2010 7/34 There is another top address in Darmstadt! Immunoassay buffer AppliCoat Plate Stabilizer o for the long-term storage of precoated ELISA plates o preservation of immobilized antibodies and proteins o for reproducible results (uniformly coated plates of 1 lot)

8. AppliChem © 2010 8/34 There is another top address in Darmstadt! Immunoassay buffer AppliCoat Plate Stabilizer o easy-to-use: simply apply like a 2 nd blocking step

9. AppliChem © 2010 9/34 There is another top address in Darmstadt! Immunoassay buffer days of storage at 37°C OD 450nm AppliCoat Plate Stabilizer o Stress test at 37°C for 84 days: prolonged shelf-life

10. AppliChem © 2010 10/34 There is another top address in Darmstadt! Immunoassay buffer Related products o Stripping buffers o Washing buffers o Coating buffers o Sample buffers o Blocking Buffers II and III o Transfer membranes o Size marker

11. AppliChem © 2010 11/34 There is another top address in Darmstadt! Immunoassay buffer Literature