1. Disturbing (polymers and) proteins with Atomic Force Microscopy José L. Toca-Herrera ETSEQ, 02/06/2004

2. DNA, proteins are polymers... with AFM you can see them (nano)structure! and most important you can measure inter and intramolecular forces Is that all you can do??

3. The AFM Experiment: imaging The movements of the reflected light are "seen" by a photodiode. So the magnitude of the diode current in addition to the position of the tip on the plane leads to an image, which provides three-dimensional information regarding the sample

4. The AFM Experiment: pulling

5. Cantilevers (and tips) Different types and shapes different mechanical properties

6. The cantilever is the sensor of the AFM Mechanical properties can tell us something about interfacial phenomena

7. What does AFM have to offer? Alternative to conventional methods of denaturation (e.g. heat, acid, chemical denaturant) and kinetic reactions in general Single molecule experiment: measurement of inter and intra molecular forces Well defined reaction coordinate Direct comparison with all-atom MD and Monte Carlo simulations

8. Something more to offer? Alternative to conventional methods of “take a chance on me”: other microscopies like TEM, SEM…sub-nanometer resolution You can follow processes on-line such as structural changes NOT only single molecule: attach a colloidal particle You can work with(in) solvents: biological condition, etc…

9. S-layer on PAA - Silicon wafer + PEI + (PAA + PDADMAC) 2 + PAA

10. Switching off/on S-layers : water-EtOH mixtures - Hydrophilic Silicon as substrate - 80% vol EtOH unfolds the protein...after buffer treatment we get it back!

11. Modeling: Monte-Carlo Simulations (I) Folding and Unfolding rates given by

12. In the Beginning there was Titin…

13. Interpreting traces Unfolding proteins by AFM is a kinetic measurement: average unfolding force depends on pulling speed. Average unfolding rates can be estimated by Monte-Carlo simulation.

14. Conservative, non-disruptive, significantly destabilising A’-strand V13A G-strand V86A F strand F73L C-D loop L41A Choice of mutants is critical A-strand V4A

15. Evidence from mutation When we pull a protein with a destabilising mutation in the A strand (V4A) it does not affect the unfolding forces at all

16. MD simulation supports this pattern

17. Carbohydrate-protein interaction a)Attracttive forces appear when the tip approaches the surface covered with S- layers. b)By cantilever retraction large adhesion forces with different peaks can be measured (see histogram, left). After pulling a length of about 120-140 nm, here we are measuring carbohydraete/protein forces... elasticity of protein domains can also be detected (in general lower than 300 pN).

18. Force between cell walls -SiO x coated particle glued (UHU-plus endfest 300 ) on rectangular cantilever -Adhesion force about 1mN/m -Conexion with SFA

19. Conclusions AFM is a suitable tool to investigate polymer elasticity, binding kinetics, molecular recognition and surface properties… and …this includes everything related with biology, surface chemistry, physics and material science AFMs are in continuos development: hardware and software…almost everybody can contribute!