2. Gihan E-H Gawish, MSc, PhD Ass. Professor Molecular Genetics and Clinical Biochemistry Molecular Genetics and Clinical BiochemistryKSU 9 TH WEEK

3.  After replication,  each DNA molecule= one old strand and one new strand.  After replication,  each DNA molecule= one old strand and one new strand.

4. Three possible models Correct

5.  Watson and Crick’s structure predicted that base-pairing would guide semi-conservative replication.  Matthew Meselson and Franklin Stahl tested this prediction by labeling DNA with 15N (heavy isotope).  Watson and Crick’s structure predicted that base-pairing would guide semi-conservative replication.  Matthew Meselson and Franklin Stahl tested this prediction by labeling DNA with 15N (heavy isotope).

6. Animation

7. Click to find the attached file Click to Exam Yourself Click to Exam Yourself click to solve the quiz in this animation DNA Replication Fork Animation

8.  3’OH of growing chain forms covalent bond with phosphate of dNTP.  DNA polymerases can only add nucleotides to 3’ end (5’—>3’ direction of synth.

9.  1) Template to specify complementary strand.  2) Primer (DNA or RNA) bound to template by base pairing. No DNA pol. can start synth. without a primer!!  3) dNTPs

10. Heat to 95C 5’-GATCTTAGC-3’ 3’-CTAGAATCG-5’ Cool to 70 C When cooled, base-pairs find each other and become double-stranded (renature, reanneal, or hybridize). 5’-GATCTTAGC-3’ 3’-CTAGAATCG-5’ DNA can be denatured and reannealed:

11.  Uses DNA replication to amplify tiny amounts of DNA to give an amount large enough to be analyzed. Requires:  1) Small amount of template.  2) Specific primers that bind the template.  3) dNTPs.  4) A thermostable DNA polymerase.  Uses DNA replication to amplify tiny amounts of DNA to give an amount large enough to be analyzed. Requires:  1) Small amount of template.  2) Specific primers that bind the template.  3) dNTPs.  4) A thermostable DNA polymerase.